Literature DB >> 18664613

Eleven golden rules of quantitative RT-PCR.

Michael K Udvardi, Tomasz Czechowski, Wolf-Rüdiger Scheible.   

Abstract

Mesh:

Year:  2008        PMID: 18664613      PMCID: PMC2518243          DOI: 10.1105/tpc.108.061143

Source DB:  PubMed          Journal:  Plant Cell        ISSN: 1040-4651            Impact factor:   11.277


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  6 in total

1.  Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.

Authors:  Christian Ramakers; Jan M Ruijter; Ronald H Lekanne Deprez; Antoon F M Moorman
Journal:  Neurosci Lett       Date:  2003-03-13       Impact factor: 3.046

2.  Properties of the reverse transcription reaction in mRNA quantification.

Authors:  Anders Ståhlberg; Joakim Håkansson; Xiaojie Xian; Henrik Semb; Mikael Kubista
Journal:  Clin Chem       Date:  2004-01-15       Impact factor: 8.327

3.  Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.

Authors:  Michael W Pfaffl; Ales Tichopad; Christian Prgomet; Tanja P Neuvians
Journal:  Biotechnol Lett       Date:  2004-03       Impact factor: 2.461

4.  Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.

Authors:  Tomasz Czechowski; Rajendra P Bari; Mark Stitt; Wolf-Rüdiger Scheible; Michael K Udvardi
Journal:  Plant J       Date:  2004-04       Impact factor: 6.417

5.  Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.

Authors:  Tomasz Czechowski; Mark Stitt; Thomas Altmann; Michael K Udvardi; Wolf-Rüdiger Scheible
Journal:  Plant Physiol       Date:  2005-09       Impact factor: 8.340

6.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583

  6 in total
  253 in total

1.  The expression levels of DNMT3a/3b and their relationship with meat quality in beef cattle.

Authors:  Xiangyu Guo; Xuan Liu; Xianzhou Xu; Meng Wu; Xu Zhang; Qiang Li; Wenjiao Liu; Yi Zhang; Yachun Wang; Ying Yu
Journal:  Mol Biol Rep       Date:  2011-12-23       Impact factor: 2.316

2.  Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions.

Authors:  José V Die; Belén Román; Salvador Nadal; Clara I González-Verdejo
Journal:  Planta       Date:  2010-04-09       Impact factor: 4.116

3.  The unified ICE-CBF pathway provides a transcriptional feedback control of freezing tolerance during cold acclimation in Arabidopsis.

Authors:  Ye Seul Kim; Minyoung Lee; Jae-Hyung Lee; Hyo-Jun Lee; Chung-Mo Park
Journal:  Plant Mol Biol       Date:  2015-08-27       Impact factor: 4.076

4.  Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.

Authors:  Ronei Dorneles Machado; Ana Paula Christoff; Guilherme Loss-Morais; Márcia Margis-Pinheiro; Rogério Margis; Ana Paula Körbes
Journal:  Plant Cell Rep       Date:  2015-02-27       Impact factor: 4.570

Review 5.  Functional analysis of transcription factors in Arabidopsis.

Authors:  Nobutaka Mitsuda; Masaru Ohme-Takagi
Journal:  Plant Cell Physiol       Date:  2009-05-28       Impact factor: 4.927

6.  Real-time quantitative RT-PCR: design, calculations, and statistics.

Authors:  Ivo Rieu; Stephen J Powers
Journal:  Plant Cell       Date:  2009-04-24       Impact factor: 11.277

7.  Validation of reference transcripts in strawberry (Fragaria spp.).

Authors:  Maureen A Clancy; Hernan G Rosli; Srikar Chamala; W Brad Barbazuk; P Marcos Civello; Kevin M Folta
Journal:  Mol Genet Genomics       Date:  2013-09-24       Impact factor: 3.291

8.  Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes.

Authors:  Kai Graeber; Ada Linkies; Kerstin Müller; Andrea Wunchova; Anita Rott; Gerhard Leubner-Metzger
Journal:  Plant Mol Biol       Date:  2009-12-15       Impact factor: 4.076

9.  Carbonic anhydrases are upstream regulators of CO2-controlled stomatal movements in guard cells.

Authors:  Honghong Hu; Aurélien Boisson-Dernier; Maria Israelsson-Nordström; Maik Böhmer; Shaowu Xue; Amber Ries; Jan Godoski; Josef M Kuhn; Julian I Schroeder
Journal:  Nat Cell Biol       Date:  2009-12-13       Impact factor: 28.824

10.  Chloroplastic phosphoadenosine phosphosulfate metabolism regulates basal levels of the prohormone jasmonic acid in Arabidopsis leaves.

Authors:  Víctor M Rodríguez; Aurore Chételat; Paul Majcherczyk; Edward E Farmer
Journal:  Plant Physiol       Date:  2010-01-06       Impact factor: 8.340

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