BACKGROUND: Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces. METHODS: RNase was added into culture media of HT-29 cells and fecal homogenates. The relative quantifications of miRNA were analyzed by real-time RT-PCR. RESULTS: Cellular miRNA or exosomal miRNA were protected from RNase by the cellular membrane or the exosome; meanwhile, free miRNA was degraded immediately and completely by RNase. CONCLUSION: The present study revealed that exosome or cellular membrane could prevent RNase from degrading miRNA inside the exosome or cells even in a dreadful condition, as in feces.
BACKGROUND: Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces. METHODS: RNase was added into culture media of HT-29 cells and fecal homogenates. The relative quantifications of miRNA were analyzed by real-time RT-PCR. RESULTS: Cellular miRNA or exosomal miRNA were protected from RNase by the cellular membrane or the exosome; meanwhile, free miRNA was degraded immediately and completely by RNase. CONCLUSION: The present study revealed that exosome or cellular membrane could prevent RNase from degrading miRNA inside the exosome or cells even in a dreadful condition, as in feces.
Keywords:
Exosome; cancer screening; colonocyte; fecal miRNA test; miRNA
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