OBJECTIVE: To evaluate the relationship between aneuploidy and timing of blastocyst formation. DESIGN: Historical cohort study. SETTING: Private IVF clinic. PATIENT(S): Ninety-four couples undergoing IVF treatment in combination with chromosomal screening of embryos. The mean maternal age was 39.2 years and average number of embryos per patient 5.3. INTERVENTION(S): A total of 530 embryos were biopsied on day 3 and underwent chromosome screening with microarray-based comparative genomic hybridization. MAIN OUTCOME MEASURE(S): Effect of day of embryo blastulation and morphologic grade on aneuploidy rate. RESULT(S): Day 5 morulas that progressed to blastocysts on day 6 were significantly less likely to be aneuploid (79.8%) than day 5 morulas that did not progress to blastocysts (92.9%). However, there was no significant difference in aneuploidy rates when embryos that became blastocysts on day 5 were directly compared with embryos that became blastocysts on day 6. CONCLUSION(S): Delayed blastulation is not associated with increased aneuploidy rates, but absence of blastulation is associated with increased aneuploidy. Therefore, we conclude that when choosing a morula for transfer on day 5, there may be a benefit in waiting an extra day for the possibility of blastulation to occur.
OBJECTIVE: To evaluate the relationship between aneuploidy and timing of blastocyst formation. DESIGN: Historical cohort study. SETTING: Private IVF clinic. PATIENT(S): Ninety-four couples undergoing IVF treatment in combination with chromosomal screening of embryos. The mean maternal age was 39.2 years and average number of embryos per patient 5.3. INTERVENTION(S): A total of 530 embryos were biopsied on day 3 and underwent chromosome screening with microarray-based comparative genomic hybridization. MAIN OUTCOME MEASURE(S): Effect of day of embryo blastulation and morphologic grade on aneuploidy rate. RESULT(S): Day 5 morulas that progressed to blastocysts on day 6 were significantly less likely to be aneuploid (79.8%) than day 5 morulas that did not progress to blastocysts (92.9%). However, there was no significant difference in aneuploidy rates when embryos that became blastocysts on day 5 were directly compared with embryos that became blastocysts on day 6. CONCLUSION(S): Delayed blastulation is not associated with increased aneuploidy rates, but absence of blastulation is associated with increased aneuploidy. Therefore, we conclude that when choosing a morula for transfer on day 5, there may be a benefit in waiting an extra day for the possibility of blastulation to occur.
Authors: Mae Wu Healy; Meghan Yamasaki; George Patounakis; Kevin S Richter; Kate Devine; Alan H DeCherney; Micah J Hill Journal: Hum Reprod Date: 2016-12-16 Impact factor: 6.918
Authors: Jonathan D Kort; Ruth B Lathi; Kathleen Brookfield; Valerie L Baker; Qianying Zhao; Barry R Behr Journal: J Assist Reprod Genet Date: 2015-04-29 Impact factor: 3.412