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Literature DB >> 22782065

Templated chemistry for monitoring damage and repair directly in duplex DNA.

Seoung Ho Lee¹, Shenliang Wang, Eric T Kool.

Abstract

We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA Probes
DNA

Year: 2012 PMID： 22782065 PMCID： PMC3417126 DOI： 10.1039/c2cc34060g

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

48 in total

Review 1. Abasic DNA structure, reactivity, and recognition.

Authors: J Lhomme; J F Constant; M Demeunynck
Journal: Biopolymers Date: 1999 Impact factor: 2.505

2. Site-directed spin-labeling of nucleic acids by click chemistry: detection of abasic sites in duplex DNA by EPR spectroscopy.

Authors: Ulla Jakobsen; Sandip A Shelke; Stefan Vogel; Snorri Th Sigurdsson
Journal: J Am Chem Soc Date: 2010-08-04 Impact factor: 15.419

Review 3. The intricate structural chemistry of base excision repair machinery: implications for DNA damage recognition, removal, and repair.

Authors: Kenichi Hitomi; Shigenori Iwai; John A Tainer
Journal: DNA Repair (Amst) Date: 2007-01-08

4. Selective detection and quantification of oxidized abasic lesions in DNA.

Authors: Shanta Dhar; Tetsuya Kodama; Marc M Greenberg
Journal: J Am Chem Soc Date: 2007-06-26 Impact factor: 15.419

5. Construction of highly reactive probes for abasic site detection by introduction of an aromatic and a guanidine residue into an aminooxy group.

Authors: Naoshi Kojima; Toshie Takebayashi; Akiko Mikami; Eiko Ohtsuka; Yasuo Komatsu
Journal: J Am Chem Soc Date: 2009-09-23 Impact factor: 15.419

6. O-(fluoresceinylmethyl)hydroxylamine (OFMHA): a fluorescent reagent for detection of damaged nucleic acids.

Authors: M Adamczyk; P G Mattingly; J A Moore
Journal: Bioorg Med Chem Lett Date: 1998-12-15 Impact factor: 2.823

7. Oligodeoxynucleotides containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic endonucleases.

Authors: M Takeshita; C N Chang; F Johnson; S Will; A P Grollman
Journal: J Biol Chem Date: 1987-07-25 Impact factor: 5.157

Templated chemistry for monitoring damage and repair directly in duplex DNA.

Review 1. Abasic DNA structure, reactivity, and recognition.

2. Site-directed spin-labeling of nucleic acids by click chemistry: detection of abasic sites in duplex DNA by EPR spectroscopy.

Review 3. The intricate structural chemistry of base excision repair machinery: implications for DNA damage recognition, removal, and repair.

4. Selective detection and quantification of oxidized abasic lesions in DNA.

5. Construction of highly reactive probes for abasic site detection by introduction of an aromatic and a guanidine residue into an aminooxy group.

6. O-(fluoresceinylmethyl)hydroxylamine (OFMHA): a fluorescent reagent for detection of damaged nucleic acids.

7. Oligodeoxynucleotides containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic endonucleases.

8. Turning On uracil-DNA glycosylase using a pyrene nucleotide switch.

Review 9. Structure and function in the uracil-DNA glycosylase superfamily.

10. Efficient nucleic acid detection by templated reductive quencher release.

1. A Fluorescent Probe to Measure DNA Damage and Repair.