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Literature DB >> 22697363

A facile method to synthesize histones with posttranslational modification mimics.

Zhiyong U Wang¹, Yane-Shih Wang, Pei-Jing Pai, William K Russell, David H Russell, Wenshe R Liu.

Abstract

Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

Entities: CellLine Chemical Disease Gene Mutation Species

Mesh：

Substances：
Histones
Serine
Lysine

Year: 2012 PMID： 22697363 PMCID： PMC3448024 DOI： 10.1021/bi300535a

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

14 in total

1. The role of DNA methylation in mammalian epigenetics.

Authors: P A Jones; D Takai
Journal: Science Date: 2001-08-10 Impact factor: 47.728

Review 2. Cellular memory and the histone code.

Authors: Bryan M Turner
Journal: Cell Date: 2002-11-01 Impact factor: 41.582

Review 3. 'Top down' protein characterization via tandem mass spectrometry.

Authors: Gavin E Reid; Scott A McLuckey
Journal: J Mass Spectrom Date: 2002-07 Impact factor: 1.982

Review 4. Chromatin modifications and their function.

Authors: Tony Kouzarides
Journal: Cell Date: 2007-02-23 Impact factor: 41.582

5. Histone H4-K16 acetylation controls chromatin structure and protein interactions.

Authors: Michael Shogren-Knaak; Haruhiko Ishii; Jian-Min Sun; Michael J Pazin; James R Davie; Craig L Peterson
Journal: Science Date: 2006-02-10 Impact factor: 47.728

6. Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins.

Authors: Jiantao Guo; Jiangyun Wang; Jong Seok Lee; Peter G Schultz
Journal: Angew Chem Int Ed Engl Date: 2008 Impact factor: 15.336

7. Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation.

Authors: Robert K McGinty; Jaehoon Kim; Champak Chatterjee; Robert G Roeder; Tom W Muir
Journal: Nature Date: 2008-04-30 Impact factor: 49.962

8. Ion mobility-mass spectrometry (IM-MS) for top-down proteomics: increased dynamic range affords increased sequence coverage.

Authors: Nathanael F Zinnel; Pei-Jing Pai; David H Russell
Journal: Anal Chem Date: 2012-03-22 Impact factor: 6.986

9. Convergent synthesis of peptide conjugates using dehydroalanines for chemoselective ligations.

Authors: Y Zhu; W A van der Donk
Journal: Org Lett Date: 2001-04-19 Impact factor: 6.005

10. Facile conversion of cysteine and alkyl cysteines to dehydroalanine on protein surfaces: versatile and switchable access to functionalized proteins.

Authors: Gonçalo J L Bernardes; Justin M Chalker; James C Errey; Benjamin G Davis
Journal: J Am Chem Soc Date: 2008-03-22 Impact factor: 15.419

16 in total

A facile method to synthesize histones with posttranslational modification mimics.

1. The role of DNA methylation in mammalian epigenetics.

Review 2. Cellular memory and the histone code.

Review 3. 'Top down' protein characterization via tandem mass spectrometry.

Review 4. Chromatin modifications and their function.

5. Histone H4-K16 acetylation controls chromatin structure and protein interactions.

6. Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins.

7. Chemically ubiquitylated histone H2B stimulates hDot1L-mediated intranucleosomal methylation.

8. Ion mobility-mass spectrometry (IM-MS) for top-down proteomics: increased dynamic range affords increased sequence coverage.

9. Convergent synthesis of peptide conjugates using dehydroalanines for chemoselective ligations.

10. Facile conversion of cysteine and alkyl cysteines to dehydroalanine on protein surfaces: versatile and switchable access to functionalized proteins.

1. Aminoacylation of tRNA 2'- or 3'-hydroxyl by phosphoseryl- and pyrrolysyl-tRNA synthetases.

2. Genetic encoding of 2-aryl-5-carboxytetrazole-based protein photo-cross-linkers.

Review 3. Histones: at the crossroads of peptide and protein chemistry.

4. Proteins with Site-Specific Lysine Methylation.

Review 5. Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Review 6. Chemical biology approaches for studying posttranslational modifications.

Review 7. Dehydroamino acids: chemical multi-tools for late-stage diversification.

8. Genetically Introducing Biochemically Reactive Amino Acids Dehydroalanine and Dehydrobutyrine in Proteins.

9. Chemical and biological tools for the preparation of modified histone proteins.

10. Exploring the substrate range of wild-type aminoacyl-tRNA synthetases.