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Literature DB >> 2268165

Specific plate assay for bacterial heparinase.

Abstract

A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of recombinant heparinase and to identify Flavobacterium heparinum mutants expressing heparinase constitutively.

Entities: Chemical Species

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Year: 1990 PMID： 2268165 PMCID： PMC185029 DOI： 10.1128/aem.56.11.3593-3594.1990

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

1. Production of heparinase by Bacteroides.

Authors: B M GESNER; C R JENKIN
Journal: J Bacteriol Date: 1961-04 Impact factor: 3.490

2. Heparinase activity in lesion of periodontal diseases.

Authors: T Nakamura; Y Suginaka; I Takazoe
Journal: Bull Tokyo Dent Coll Date: 1976-08

3. The enzymatic degradation of heparin and heparitin sulfate. I. The fractionation of a crude heparinase from flavobacteria.

Authors: A Linker; P Hovingh
Journal: J Biol Chem Date: 1965-10 Impact factor: 5.157

4. Sequential degradation of heparin in Flavobacterium heparinum. Purification and properties of five enzymes involved in heparin degradation.

Authors: C P Dietrich; M E Silva; Y M Michelacci
Journal: J Biol Chem Date: 1973-09-25 Impact factor: 5.157

5. Hydrolytic enzymes of anaerobic bacteria isolated from human infections.

Authors: E K Steffen; D J Hentges
Journal: J Clin Microbiol Date: 1981-08 Impact factor: 5.948

6. Differential anticoagulant activity of heparin fragments prepared using microbial heparinase.

Authors: R J Linhardt; A Grant; C L Cooney; R Langer
Journal: J Biol Chem Date: 1982-07-10 Impact factor: 5.157

7. Flavobacterium heparinum 3-O-sulphatase for N-substituted glucosamine 3-O-sulphate.

Authors: J S Bruce; M W McLean; W F Long; F B Williamson
Journal: Eur J Biochem Date: 1985-04-15

8. Flavobacterium heparinum 2-O-sulphatase for 2-O-sulphato-delta 4,5-glycuronate-terminated oligosaccharides from heparin.

Authors: M W McLean; J S Bruce; W F Long; F B Williamson
Journal: Eur J Biochem Date: 1984-12-17

9. Heparinase production by Flavobacterium heparinum.

Authors: P M Galliher; C L Cooney; R Langer; R J Linhardt
Journal: Appl Environ Microbiol Date: 1981-02 Impact factor: 4.792

10. Purification and characterization of heparinase from Flavobacterium heparinum.

Authors: V C Yang; R J Linhardt; H Bernstein; C L Cooney; R Langer
Journal: J Biol Chem Date: 1985-02-10 Impact factor: 5.157

7 in total

1. Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum.

Authors: H Su; F Blain; R A Musil; J J Zimmermann; K Gu; D C Bennett
Journal: Appl Environ Microbiol Date: 1996-08 Impact factor: 4.792

2. Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum.

Authors: A L Tkalec; D Fink; F Blain; G Zhang-Sun; M Laliberte; D C Bennett; K Gu; J J Zimmermann; H Su
Journal: Appl Environ Microbiol Date: 2000-01 Impact factor: 4.792

3. Expression system for high levels of GAG lyase gene expression and study of the hepA upstream region in Flavobacterium heparinum.

Authors: Françoise Blain; A Lydia Tkalec; Zhongqi Shao; Catherine Poulin; Marc Pedneault; Kangfu Gu; Bernhard Eggimann; Joe Zimmermann; Hongsheng Su
Journal: J Bacteriol Date: 2002-06 Impact factor: 3.490