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Literature DB >> 22660739

Multiview light-sheet microscope for rapid in toto imaging.

Uros Krzic¹, Stefan Gunther, Timothy E Saunders, Sebastian J Streichan, Lars Hufnagel.

Abstract

We present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.

Entities: Species

Mesh：

Year: 2012 PMID： 22660739 DOI： 10.1038/nmeth.2064

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

16 in total

Multiview light-sheet microscope for rapid in toto imaging.

1. Optical sectioning deep inside live embryos by selective plane illumination microscopy.

2. Live-cell 3D super-resolution imaging in thick biological samples.

3. Software for bead-based registration of selective plane illumination microscopy data.

4. Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase.

5. Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.

6. Deep and fast live imaging with two-photon scanned light-sheet microscopy.

7. Orthogonal-plane fluorescence optical sectioning: three-dimensional imaging of macroscopic biological specimens.

8. Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain.

9. Integration of contractile forces during tissue invagination.

10. Fast, high-contrast imaging of animal development with scanned light sheet-based structured-illumination microscopy.

1. Efficient processing and analysis of large-scale light-sheet microscopy data.

2. 3D fluorescence anisotropy imaging using selective plane illumination microscopy.

3. Whole-animal functional and developmental imaging with isotropic spatial resolution.

4. Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms.

5. CLARITY for mapping the nervous system.

Review 6. A self-organization framework for symmetry breaking in the mammalian embryo.

7. Two views on light sheets.

8. Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos.

Review 9. Faster fluorescence microscopy: advances in high speed biological imaging.

10. Unified quantitative characterization of epithelial tissue development.