BACKGROUND: There are multiple potential regulators of neonatal vitamin D status of environmental, genetic, and epigenetic origins. The relation between these factors and circulating neonatal vitamin D has yet to be fully characterized. OBJECTIVE: The aim of this study was to examine the relative contribution of genetic factors, maternal circulating 25-hydroxyvitamin D [25(OH)D] concentrations, and the placental methylation level of the gene that encodes the primary catabolic enzyme of active vitamin D [25(OH)D-24-hydroxylase encoded by CYP24A1] to neonatal 25(OH)D concentrations. DESIGN: We used the classical twin study design to determine the genetic contribution to neonatal 25(OH)D. A total of 86 twin pairs (32 monozygotic and 54 dizygotic twin pairs) were included in this study. Serum 25(OH)D was measured by using a 25(OH)D kit. CYP24A1 promoter DNA methylation was measured by means of matrix-assisted laser desorption time-of-flight mass spectrometry. RESULTS: Maternal and neonatal 25(OH)D showed a strong association (R² = 0.19). Monozygotic and dizygotic within-pair serum 25(OH)D correlations were similar (R² = 0.71 and 0.67, respectively), which suggested no genetic effect. Placental CYP24A1 methylation did not show an association with maternal or neonatal 25(OH)D concentrations. CONCLUSIONS: Our results suggest that maternal circulating 25(OH)D is the most significant regulator of neonatal circulating 25(OH)D concentrations, with underlying genetic factors playing a limited role. The placental methylation of the CYP24A1 promoter appears subject to a genetic influence, although no evidence of a relation between the methylation level of this gene and circulating maternal or neonatal 25(OH)D was apparent.
BACKGROUND: There are multiple potential regulators of neonatal vitamin D status of environmental, genetic, and epigenetic origins. The relation between these factors and circulating neonatal vitamin D has yet to be fully characterized. OBJECTIVE: The aim of this study was to examine the relative contribution of genetic factors, maternal circulating 25-hydroxyvitamin D [25(OH)D] concentrations, and the placental methylation level of the gene that encodes the primary catabolic enzyme of active vitamin D [25(OH)D-24-hydroxylase encoded by CYP24A1] to neonatal 25(OH)D concentrations. DESIGN: We used the classical twin study design to determine the genetic contribution to neonatal 25(OH)D. A total of 86 twin pairs (32 monozygotic and 54 dizygotic twin pairs) were included in this study. Serum 25(OH)D was measured by using a 25(OH)D kit. CYP24A1 promoter DNA methylation was measured by means of matrix-assisted laser desorption time-of-flight mass spectrometry. RESULTS: Maternal and neonatal 25(OH)D showed a strong association (R² = 0.19). Monozygotic and dizygotic within-pair serum 25(OH)D correlations were similar (R² = 0.71 and 0.67, respectively), which suggested no genetic effect. Placental CYP24A1 methylation did not show an association with maternal or neonatal 25(OH)D concentrations. CONCLUSIONS: Our results suggest that maternal circulating 25(OH)D is the most significant regulator of neonatal circulating 25(OH)D concentrations, with underlying genetic factors playing a limited role. The placental methylation of the CYP24A1 promoter appears subject to a genetic influence, although no evidence of a relation between the methylation level of this gene and circulating maternal or neonatal 25(OH)D was apparent.
Authors: Maria C Magnus; Lars C Stene; Siri E Håberg; Per Nafstad; Hein Stigum; Stephanie J London; Wenche Nystad Journal: Paediatr Perinat Epidemiol Date: 2013-08-20 Impact factor: 3.980
Authors: Steffen U Thorsen; Karl Mårild; Sjurdur F Olsen; Klaus K Holst; German Tapia; Charlotta Granström; Thorhallur I Halldorsson; Arieh S Cohen; Margaretha Haugen; Marika Lundqvist; Torild Skrivarhaug; Pål R Njølstad; Geir Joner; Per Magnus; Ketil Størdal; Jannet Svensson; Lars C Stene Journal: Am J Epidemiol Date: 2018-06-01 Impact factor: 4.897
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Authors: E Morales; A Rodriguez; D Valvi; C Iñiguez; A Esplugues; J Vioque; L S Marina; A Jiménez; M Espada; C R Dehli; A Fernández-Somoano; M Vrijheid; J Sunyer Journal: Int J Obes (Lond) Date: 2014-09-05 Impact factor: 5.095
Authors: Mehmet Nevzat Cizmeci; Mehmet Kenan Kanburoglu; Ahmet Zulfikar Akelma; Abdullah Ayyildiz; Irem Kutukoglu; Dilara Dilsad Malli; Mustafa Mansur Tatli Journal: Eur J Pediatr Date: 2014-12-12 Impact factor: 3.183