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Literature DB >> 226362

Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.

Abstract

Chromatin was assembled in vitro from simian virus 40 DNA form I and the calf-thymus four histones H2A, H2B, H3 and H4. Transcription with calf thymus RNA polymerases A and B (I and II) was greatly inhibited. Nucleosomes were found to inhibit both RNA chain initiation and elongation. The inhibition of elongation could be relieved by increasing ionic strength, suggesting that electrostatic interactions between histone octamer and DNA have to be broken for RNA polymerase to transcribe DNA organised into nucleosomes.

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Year: 1979 PMID： 226362 DOI： 10.1111/j.1432-1033.1979.tb13191.x

Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956

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Cited

20 in total

1. Stability of a human SWI-SNF remodeled nucleosomal array.

Authors: J R Guyon; G J Narlikar; E K Sullivan; R E Kingston
Journal: Mol Cell Biol Date: 2001-02 Impact factor: 4.272

2. Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes.

Authors: M J Fedor; R D Kornberg
Journal: Mol Cell Biol Date: 1989-04 Impact factor: 4.272

3. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo.

Authors: G Sebastiaan Winkler; Arnold Kristjuhan; Hediye Erdjument-Bromage; Paul Tempst; Jesper Q Svejstrup
Journal: Proc Natl Acad Sci U S A Date: 2002-03-19 Impact factor: 11.205

Review 4. Chromatin and transcription in yeast.

Authors: Oliver J Rando; Fred Winston
Journal: Genetics Date: 2012-02 Impact factor: 4.562

5. Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae.

Authors: N J Krogan; J F Greenblatt
Journal: Mol Cell Biol Date: 2001-12 Impact factor: 4.272

Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.

1. Stability of a human SWI-SNF remodeled nucleosomal array.

2. Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes.

3. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo.

Review 4. Chromatin and transcription in yeast.

5. Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae.

6. An examination of models for chromatin transcription.

7. Evidence for transcriptional regulation of the myosin heavy chain gene during myogenesis.

8. Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins.

9. The chromatin structure of an actively expressed, single copy yeast gene.

10. The Elp3 subunit of human Elongator complex is functionally similar to its counterpart in yeast.