Literature DB >> 2254034

Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR.

T J Brickman1, M Boesman-Finkelstein, R A Finkelstein, M A McIntosh.   

Abstract

The ctx operons from the Vibrio cholerae El Tor strain 3083 and its CtxA- derivative Texas Star-SR were cloned, and their nucleotide sequences were compared. A single missense mutation in the Texas Star-SR ctxA cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid CtxA precursor was identified. Immunoblot analysis detected the mutant CtxA intracellularly early in the culture cycle but not extracellularly at any growth stage.

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Year:  1990        PMID： 2254034      PMCID： PMC313787          DOI： 10.1128/iai.58.12.4142-4144.1990

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  14 in total

1.  Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin.

Authors:  T Honda; R A Finkelstein
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

2.  Volunteer studies of deletion mutants of Vibrio cholerae O1 prepared by recombinant techniques.

Authors:  M M Levine; J B Kaper; D Herrington; G Losonsky; J G Morris; M L Clements; R E Black; B Tall; R Hall
Journal:  Infect Immun       Date:  1988-01       Impact factor: 3.441

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Pathogenesis of experimental cholera: biologic ativities of purified procholeragen A.

Authors:  R A Finkelstein; P Atthasampunna; M Chulasamaya; P Charunmethee
Journal:  J Immunol       Date:  1966-03       Impact factor: 5.422

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.

Authors:  J J Mekalanos; D J Swartz; G D Pearson; N Harford; F Groyne; M de Wilde
Journal:  Nature       Date:  1983 Dec 8-14       Impact factor: 49.962

7.  Procholeragenoid: an aggregated intermediate in the formation of choleragenoid.

Authors:  R A Finkelstein; K Fujita; J J LoSpalluto
Journal:  J Immunol       Date:  1971-10       Impact factor: 5.422

8.  Pathogenesis of experimental cholera: identification of choleragen (procholeragen A) by disc immunoelectrophoresis and its differentiation from cholera mucinase.

Authors:  R A Finkelstein; P Z Sobocinski; P Atthasampunna; P Charunmethee
Journal:  J Immunol       Date:  1966-07       Impact factor: 5.422

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid.

Authors:  R A Finkelstein; J J LoSpalluto
Journal:  J Exp Med       Date:  1969-07-01       Impact factor: 14.307
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  3 in total

1.  Construction and characterization of recombinant Vibrio cholerae strains producing inactive cholera toxin analogs.

Authors:  C C Häse; L S Thai; M Boesman-Finkelstein; V L Mar; W N Burnette; H R Kaslow; L A Stevens; J Moss; R A Finkelstein
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

2.  Development and testing of a nonradioactive DNA oligonucleotide probe that is specific for Vibrio cholerae cholera toxin.

Authors:  A C Wright; Y Guo; J A Johnson; J P Nataro; J G Morris
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

3.  Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment.

Authors:  R A Finkelstein; M Boesman-Finkelstein; Y Chang; C C Häse
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441
  3 in total

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