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Literature DB >> 2253935

R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1).

E Takahashi¹, T Hori, P O'Connell, M Leppert, R White.

Abstract

A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes.

Entities: Chemical Gene Species

Mesh：

Substances：
DNA Probes
Collagen

Year: 1990 PMID： 2253935 DOI： 10.1007/bf00205165

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

11 in total

1. Mapping of the human type II collagen gene (COL2A1) proximal to fra(12) (q13.1) by nonisotopic in situ hybridization.

Authors: E Takahashi; T Hori; G R Sutherland
Journal: Cytogenet Cell Genet Date: 1990

2. Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors: E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal: Proc Natl Acad Sci U S A Date: 1989-01 Impact factor: 11.205

3. Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization.

Authors: E Viegas-Péquignot; Z L Li; B Dutrillaux; F Apiou; D Paulin
Journal: Hum Genet Date: 1989-08 Impact factor: 4.132

4. Identification and characterization of the human type II collagen gene (COL2A1).

Authors: K S Cheah; N G Stoker; J R Griffin; F G Grosveld; E Solomon
Journal: Proc Natl Acad Sci U S A Date: 1985-05 Impact factor: 11.205

5. Three-way differential staining of sister chromatids in M3 chromosomes. Evidence for spontaneous sister chromatid exchanges in vitro.

Authors: H Tsuji; H Kato
Journal: Exp Cell Res Date: 1981-08 Impact factor: 3.905

6. Replication timing of genes and middle repetitive sequences.

Authors: M A Goldman; G P Holmquist; M C Gray; L A Caston; A Nag
Journal: Science Date: 1984-05-18 Impact factor: 47.728

7. Detailed cell cycle analysis in human lymphocytes; application to gamma-irradiated cells.

Authors: J Couturier; J L Antoine
Journal: Hum Genet Date: 1984 Impact factor: 4.132

8. Characterization of Giemsa dark- and light-band DNA.

Authors: G Holmquist; M Gray; T Porter; J Jordan
Journal: Cell Date: 1982-11 Impact factor: 41.582

9. Human type II collagen gene (COL2A1) assigned to chromosome 12q13.1-q13.2 by in situ hybridization with biotinylated DNA probe.

Authors: E Takahashi; T Hori; J B Lawrence; J McNeil; R H Singer; P O'Connell; M Leppert; R White
Journal: Jinrui Idengaku Zasshi Date: 1989-12

10. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line.

Authors: J B Lawrence; C A Villnave; R H Singer
Journal: Cell Date: 1988-01-15 Impact factor: 41.582

44 in total

1. Construction of comparative cytogenetic maps of the Chinese hamster to mouse, rat and human.

Authors: A Kuroiwa; K Tsuchiya; K Matsubara; T Namikawa; Y Matsuda
Journal: Chromosome Res Date: 2001 Impact factor: 5.239

2. Genomic anatomy of a premier major histocompatibility complex paralogous region on chromosome 1q21-q22.

Authors: T Shiina; A Ando; Y Suto; F Kasai; A Shigenari; N Takishima; E Kikkawa; K Iwata; Y Kuwano; Y Kitamura; Y Matsuzawa; K Sano; M Nogami; H Kawata; S Li; Y Fukuzumi; M Yamazaki; H Tashiro; G Tamiya; A Kohda; K Okumura; T Ikemura; E Soeda; N Mizuki; M Kimura; S Bahram; H Inoko
Journal: Genome Res Date: 2001-05 Impact factor: 9.043

3. Conservation of the rat X chromosome gene order in rodent species.

Authors: A Kuroiwa; K Tsuchiya; T Watanabe; H Hishigaki; E Takahashi; T Namikawa; Y Matsuda
Journal: Chromosome Res Date: 2001 Impact factor: 5.239

4. Mapping of epidermolysis bullosa simplex mutation to chromosome 12.

Authors: M Ryynänen; R G Knowlton; J Uitto
Journal: Am J Hum Genet Date: 1991-11 Impact factor: 11.025

5. Chromosome-band-specific painting: chromosome in situ suppression hybridization using PCR products from a microdissected chromosome band as a probe pool.

Authors: H X Deng; K Yoshiura; R W Dirks; N Harada; T Hirota; K Tsukamoto; Y Jinno; N Niikawa
Journal: Hum Genet Date: 1992-04 Impact factor: 4.132

Review 6. Advances in cytogenetic analysis of solid tumours.

Authors: L James; J Varley
Journal: Chromosome Res Date: 1996-11 Impact factor: 5.239

7. Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12.

Authors: M Seki; H Miyazawa; S Tada; J Yanagisawa; T Yamaoka; S Hoshino; K Ozawa; T Eki; M Nogami; K Okumura
Journal: Nucleic Acids Res Date: 1994-11-11 Impact factor: 16.971