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Literature DB >> 2251265

Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells.

M Grez¹, E Akgün, F Hilberg, W Ostertag.

Abstract

The expression of Moloney murine leukemia virus and vectors derived from it is restricted in undifferentiated mouse embryonal carcinoma and embryonal stem (ES) cells. We have developed a retroviral vector, the murine embryonic stem cell virus (MESV), that is active in embryonal carcinoma and ES cells. MESV was derived from a retroviral mutant [PCC4-cell-passaged myeloproliferative sarcoma virus (PCMV)] expressed in embryonal carcinoma cells but not in ES cells. The enhancer region of PCMV was shown to be functional in both cell types, but sequences within the 5' untranslated region of PCMV were found to restrict viral expression in ES cells. Replacement of this region by related sequences obtained from the dl-587rev retrovirus results in MESV, a modified PCMV virus that confers G418 resistance to fibroblasts and ES cells with similar efficiencies. Expression of MESV in ES cells is mediated by transcriptional regulatory elements within the 5' long terminal repeat of the viral genome.

Entities: Chemical Disease Species

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Year: 1990 PMID： 2251265 PMCID： PMC55132 DOI： 10.1073/pnas.87.23.9202

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

1. Virus infection of murine teratocarcinoma stem cell lines.

Authors: N M Teich; R A Weiss; G R Martin; D R Lowy
Journal: Cell Date: 1977-12 Impact factor: 41.582

2. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

Authors: R Mann; R C Mulligan; D Baltimore
Journal: Cell Date: 1983-05 Impact factor: 41.582

3. Independent mechanisms involved in suppression of the Moloney leukemia virus genome during differentiation of murine teratocarcinoma cells.

Authors: O Niwa; Y Yokota; H Ishida; T Sugahara
Journal: Cell Date: 1983-04 Impact factor: 41.582

4. Delayed de novo methylation in teratocarcinoma suggests additional tissue-specific mechanisms for controlling gene expression.

Authors: J W Gautsch; M C Wilson
Journal: Nature Date: 1983-01-06 Impact factor: 49.962

5. Non-function of a Moloney murine leukaemia virus regulatory sequence in F9 embryonal carcinoma cells.

Authors: E Linney; B Davis; J Overhauser; E Chao; H Fan
Journal: Nature Date: 1984 Mar 29-Apr 4 Impact factor: 49.962

6. De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells.

Authors: C L Stewart; H Stuhlmann; D Jähner; R Jaenisch
Journal: Proc Natl Acad Sci U S A Date: 1982-07 Impact factor: 11.205

7. Establishment in culture of pluripotential cells from mouse embryos.

Authors: M J Evans; M H Kaufman
Journal: Nature Date: 1981-07-09 Impact factor: 49.962

8. Two blocks in Moloney murine leukemia virus expression in undifferentiated F9 embryonal carcinoma cells as determined by transient expression assays.

Authors: G Feuer; M Taketo; R C Hanecak; H Fan
Journal: J Virol Date: 1989-05 Impact factor: 5.103

9. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.

Authors: G R Martin
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

10. Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice.

Authors: W Ostertag; K Vehmeyer; B Fagg; I B Pragnell; W Paetz; M C Le Bousse; F Smadja-Joffe; B Klein; C Jasmin; H Eisen
Journal: J Virol Date: 1980-02 Impact factor: 5.103

59 in total

Review 1. Gene therapy for the hemoglobin disorders: past, present, and future.

Authors: D A Persons; A W Nienhuis
Journal: Proc Natl Acad Sci U S A Date: 2000-05-09 Impact factor: 11.205

2. Lack of shielding of primer binding site silencer-mediated repression of an internal promoter in a retrovirus vector by the putative insulators scs, BEAD-1, and HS4.

Authors: C Modin; F S Pedersen; M Duch
Journal: J Virol Date: 2000-12 Impact factor: 5.103

3. Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro.

Authors: I Hamaguchi; N B Woods; I Panagopoulos; E Andersson; H Mikkola; C Fahlman; R Zufferey; L Carlsson; D Trono; S Karlsson
Journal: J Virol Date: 2000-11 Impact factor: 5.103

4. Retroviral expression in embryonic stem cells and hematopoietic stem cells.

Authors: S R Cherry; D Biniszkiewicz; L van Parijs; D Baltimore; R Jaenisch
Journal: Mol Cell Biol Date: 2000-10 Impact factor: 4.272

5. DNA methylation density influences the stability of an epigenetic imprint and Dnmt3a/b-independent de novo methylation.

Authors: Matthew C Lorincz; Dirk Schübeler; Shauna R Hutchinson; David R Dickerson; Mark Groudine
Journal: Mol Cell Biol Date: 2002-11 Impact factor: 4.272