| Literature DB >> 22481628 |
Xin Luo1, Min Feng, Shirong Pan, Yuting Wen, Wei Zhang, Chuanbin Wu.
Abstract
Polyethylenimine (PEI) is an efficient cationic polymer for gene delivery, but defective in biocompatibility. In this study, we developed two different strategies to shield the positively charged PEI/DNA complexes: PEGylation and lipid coating. The physicochemical properties, cytotoxicity and transfection efficiency of the two gene delivery systems were investigated. Both PEGylation and lipid coating succeeded in reducing the zeta-potential of the complexes. Lipid-coated PEI/DNA complexes (LPD complexes) and PEI/DNA complexes exhibited similar cytotoxicity, whereas PEG-PEI/DNA complexes showed lower cytotoxicity, especially at high N/P ratios. LPD complexes were less efficient in transfection compared to PEG-PEI/DNA complexes. The transfection efficiency was influenced remarkably by cytotoxicity and surface charge of the complexes. Intracellular processes studies revealed that endosomal release might be one of the rate-limiting steps in cell transfection with PEI as a gene delivery carrier.Entities:
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Year: 2012 PMID： 22481628 DOI： 10.1007/s10856-012-4632-4
Source DB: PubMed Journal: J Mater Sci Mater Med ISSN： 0957-4530 Impact factor: 3.896