| Literature DB >> 19838772 |
Xin Luo1, Shirong Pan, Min Feng, Yuting Wen, Wei Zhang.
Abstract
Polyethylenimine (PEI) is one of the most widely investigated cationic polymers for gene delivery. However, PEI/DNA complexes are unstable and tend to aggregate. PEGylation was used to improve the stability. The stability of polymer/DNA complexes was investigated including complexation stability, aggregation stability, sedimentation stability, and nuclease stability. PEI25K/DNA complexes were liable to aggregate to large particles (500-700 nm). The aggregation was proved to be induced by phosphate anion. In the medium without phosphate anion, aggregation was prevented by electrostatic repulsion. Owing to more efficient steric repulsion, PEG2 and PEG5K excelled PEG750 in facilitating copolymers to form stable small polyplexes (below 100 nm) without aggregation regardless of phosphate anion. The steric repulsion predominated over electrostatic repulsion in stabilization.Entities:
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Year: 2009 PMID： 19838772 DOI： 10.1007/s10856-009-3903-1
Source DB: PubMed Journal: J Mater Sci Mater Med ISSN： 0957-4530 Impact factor: 3.896