| Literature DB >> 22466793 |
Fernán Federici1, Lionel Dupuy, Laurent Laplaze, Marcus Heisler, Jim Haseloff.
Abstract
We present the coupled use of specifically localized fluorescent gene markers and image processing for automated quantitative analysis of cell growth and genetic activity across living plant tissues. We used fluorescent protein markers to identify cells, create seeds and boundaries for the automatic segmentation of cell geometries and ratiometrically measure gene expression cell by cell in Arabidopsis thaliana.Entities:
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Year: 2012 PMID: 22466793 DOI: 10.1038/nmeth.1940
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547