| Literature DB >> 22449968 |
Junwen Liu1, Tao Yang, Ying Liu, Huali Zhang, Kangkai Wang, Meidong Liu, Guangwen Chen, Xianzhong Xiao.
Abstract
RAW264.7 macrophages and human peripheral blood mononuclear cells were treated with LPS to determine the expression of KLF4 and release of IL-1β. A full-length cDNA or short interference RNA of KLF4 was transfected into RAW264.7 macrophages; the expression and release of IL-1β were analyzed. The transcription and DNA binding activities of KLF4 to the IL-1β promoter were detected further. The results showed LPS treatment resulted in the increase of KLF4 level and IL-1β release; KLF4 overexpression decreased the expression of IL-1β, while KLF4 inhibition increased the expression of IL-1β; overexpression of KLF4 promoted the DNA binding activity of KLF4 to the IL-1β promoter and attenuated the transcription of IL-1β promoter, indicating an important role of KLF4 in regulating expression of IL-1β. Copyright ÂEntities:
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Year: 2012 PMID： 22449968 DOI： 10.1016/j.febslet.2012.02.003
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124