Literature DB >> 2243279

Modification of the Polaron sputter-coater unit for glow-discharge activation of carbon support films.

O Benada1, V Pokorný.   

Abstract

We describe a modification of the Polaron sputter-coater unit series 11 HD enabling activation of carbon support films for electron microscopy of macromolecules and macromolecular assemblies. The modification is simple and the device can be used in two modes, for sputter-coating of SEM samples and for glow-discharge activation of carbon support films. Examples of protein-free spreading of DNA and negative staining of bacteriophage particles on activated carbon support films are presented.

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Year:  1990        PMID： 2243279     DOI： 10.1002/jemt.1060160304

Source DB:  PubMed          Journal:  J Electron Microsc Tech        ISSN： 0741-0581


  13 in total

1.  Phage tail-like (high-molecular-weight) bacteriocins of Budvicia aquatica and Pragia fontium (Enterobacteriaceae).

Authors:  Jan Smarda; Oldrich Benada
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

2.  Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3.

Authors:  Radim Osicka; Adriana Osickova; Shakir Hasan; Ladislav Bumba; Jiri Cerny; Peter Sebo
Journal:  Elife       Date:  2015-12-09       Impact factor: 8.140

3.  The Bordetella pertussis type III secretion system tip complex protein Bsp22 is not a protective antigen and fails to elicit serum antibody responses during infection of humans and mice.

Authors:  Rodrigo Villarino Romero; Ilona Bibova; Ondrej Cerny; Branislav Vecerek; Tomas Wald; Oldrich Benada; Jana Zavadilova; Radim Osicka; Peter Sebo
Journal:  Infect Immun       Date:  2013-05-20       Impact factor: 3.441

4.  Intrinsically disordered enamel matrix protein ameloblastin forms ribbon-like supramolecular structures via an N-terminal segment encoded by exon 5.

Authors:  Tomas Wald; Adriana Osickova; Miroslav Sulc; Oldrich Benada; Alena Semeradtova; Lenka Rezabkova; Vaclav Veverka; Lucie Bednarova; Jan Maly; Pavel Macek; Peter Sebo; Ivan Slaby; Jiri Vondrasek; Radim Osicka
Journal:  J Biol Chem       Date:  2013-06-19       Impact factor: 5.157

5.  Heterologous expression, purification and characterization of nitrilase from Aspergillus niger K10.

Authors:  Ondřej Kaplan; Karel Bezouška; Ondřej Plíhal; Rüdiger Ettrich; Natallia Kulik; Ondřej Vaněk; Daniel Kavan; Oldřich Benada; Anna Malandra; Ondřej Sveda; Alicja B Veselá; Anna Rinágelová; Kristýna Slámová; Maria Cantarella; Jürgen Felsberg; Jarmila Dušková; Jan Dohnálek; Michael Kotik; Vladimír Křen; Ludmila Martínková
Journal:  BMC Biotechnol       Date:  2011-01-06       Impact factor: 2.563

6.  Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage.

Authors:  Martin Ploss; Andreas Kuhn
Journal:  BMC Microbiol       Date:  2011-09-26       Impact factor: 3.605

7.  Selective targeting of melanoma by PEG-masked protein-based multifunctional nanoparticles.

Authors:  Luca Vannucci; Elisabetta Falvo; Manuela Fornara; Patrizio Di Micco; Oldrich Benada; Jiri Krizan; Jan Svoboda; Katarina Hulikova-Capkova; Veronica Morea; Alberto Boffi; Pierpaolo Ceci
Journal:  Int J Nanomedicine       Date:  2012-03-16

8.  Novel Temperate Phages of Salmonella enterica subsp. salamae and subsp. diarizonae and Their Activity against Pathogenic S. enterica subsp. enterica Isolates.

Authors:  Lenka Mikalová; Juraj Bosák; Hana Hříbková; Daniela Dědičová; Oldřich Benada; Jan Šmarda; David Šmajs
Journal:  PLoS One       Date:  2017-01-24       Impact factor: 3.240

9.  Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells.

Authors:  Radivojka Bánová Vulić; Martina Zdurienčíková; Silvia Tyčiaková; Oldřich Benada; Mária Dubrovčáková; Ján Lakota; Ľudovít Škultéty
Journal:  J Cell Mol Med       Date:  2019-03-27       Impact factor: 5.310

10.  Francisella tularensis subsp. holarctica Releases Differentially Loaded Outer Membrane Vesicles Under Various Stress Conditions.

Authors:  Jana Klimentova; Ivona Pavkova; Lenka Horcickova; Jan Bavlovic; Olga Kofronova; Oldrich Benada; Jiri Stulik
Journal:  Front Microbiol       Date:  2019-10-10       Impact factor: 5.640
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