BACKGROUND: People with the human neutrophil antigen (HNA)-3b/3b type can make HNA-3a antibodies, which have been reported to cause immune neutropenia disorders and are especially prone to cause severe cases of transfusion-related acute lung injury. However, knowledge of HNA-3 allele frequencies outside Caucasian populations is limited. We developed a high-throughput genotyping assay and determined the HNA-3a/3b genotype frequencies in six different racial and ethnic groups. STUDY DESIGN AND METHODS: Genotyping utilized TaqMan 5' exonuclease chemistry and real-time polymerase chain reaction. A total of 742 DNA samples from six different racial and ethnic groups were genotyped for HNA-3a and HNA-3b. RESULTS: The genotyping assay showed 100% sensitivity and specificity compared to sequencing and phenotyping and had high throughput. A significant percentage of Caucasians (6.5%), Han Chinese (16%), and Asian Indians (6%) typed HNA-3b/3b, but only a small percentage of Hispanics (1%) and no African or Native Americans. CONCLUSIONS: The HNA-3 genotyping assay had high sensitivity, specificity, and sample throughput. HNA-3b/b genotype results determined for 742 individuals representing six different racial and ethnic groups showed that there could be a significant risk of producing anti-HNA-3a in Chinese, as well as in Caucasian and Asian Indian blood donor populations, but a very low risk in Hispanic, African, or Native American populations.
BACKGROUND:People with the human neutrophil antigen (HNA)-3b/3b type can make HNA-3a antibodies, which have been reported to cause immune neutropenia disorders and are especially prone to cause severe cases of transfusion-related acute lung injury. However, knowledge of HNA-3 allele frequencies outside Caucasian populations is limited. We developed a high-throughput genotyping assay and determined the HNA-3a/3b genotype frequencies in six different racial and ethnic groups. STUDY DESIGN AND METHODS: Genotyping utilized TaqMan 5' exonuclease chemistry and real-time polymerase chain reaction. A total of 742 DNA samples from six different racial and ethnic groups were genotyped for HNA-3a and HNA-3b. RESULTS: The genotyping assay showed 100% sensitivity and specificity compared to sequencing and phenotyping and had high throughput. A significant percentage of Caucasians (6.5%), Han Chinese (16%), and Asian Indians (6%) typed HNA-3b/3b, but only a small percentage of Hispanics (1%) and no African or Native Americans. CONCLUSIONS: The HNA-3 genotyping assay had high sensitivity, specificity, and sample throughput. HNA-3b/b genotype results determined for 742 individuals representing six different racial and ethnic groups showed that there could be a significant risk of producing anti-HNA-3a in Chinese, as well as in Caucasian and Asian Indian blood donor populations, but a very low risk in Hispanic, African, or Native American populations.
Authors: A Davoren; B R Curtis; I A Shulman; A F Mohrbacher; J Bux; B J Kwiatkowska; J G McFarland; R H Aster Journal: Transfusion Date: 2003-05 Impact factor: 3.157
Authors: P K Kommareddi; T S Nair; L V Thang; M M Galano; E Babu; V Ganapathy; T Kanazawa; J B McHugh; T E Carey Journal: Protein J Date: 2010-08 Impact factor: 2.371
Authors: Thankam S Nair; Kelley E Kozma; Nickoleta L Hoefling; Pavan K Kommareddi; Yo Ueda; Tzy-Wen Gong; Margaret I Lomax; Christopher D Lansford; Steven A Telian; Bulent Satar; H Alexander Arts; Hussam K El-Kashlan; Wayne E Berryhill; Yehoash Raphael; Thomas E Carey Journal: J Neurosci Date: 2004-02-18 Impact factor: 6.167
Authors: P Bierling; J Bux; B Curtis; B Flesch; L Fung; G Lucas; M Macek; E Muniz-Diaz; L Porcelijn; A Reil; U Sachs; R Schuller; N Tsuno; M Uhrynowska; S Urbaniak; N Valentin; A Wikman; B Zupanska Journal: Vox Sang Date: 2008-12-29 Impact factor: 2.144
Authors: Brian R Curtis; Nancy J Cox; Mia J Sullivan; Anuar Konkashbaev; Krista Bowens; Kirk Hansen; Richard H Aster Journal: Blood Date: 2009-12-29 Impact factor: 22.113