BACKGROUND: This study assessed the reproducibility and accuracy of 2-deoxy-2[(18)F]fluoro-D-glucose ((18)F-FDG) for non-invasive quantification of myocardial infarct size in mice by a high-resolution positron emission tomography (PET)/computed tomography (CT) system. METHODS AND RESULTS: Mice were studied by (18)F-FDG PET/CT 1 week after induction of myocardial infarction by permanent coronary occlusion or sham procedure. In a subset of mice, PET/CT was repeated 2 days apart to assess the reproducibility of infarct size measurements. Histological analysis was used as reference method to validate imaging data. The average difference in infarct size measurements between the first and the second study was -0.42% ± 2.07% (95% confidence interval -2.6 to 1.75) with a repeatability coefficient of 4.05%. At Bland-Altman analysis, the lower and upper limits of agreement between the two repeated studies were -4.46% and 3.63%, respectively, and no correlation between difference and mean was found (P = .89). The concordance correlation coefficient was 0.99 (P < .001) and the intraclass coefficient of correlation was 0.99. A high correlation between PET/CT and histology was found for measurement of infarct size (P < .001). Using Bland-Altman analysis, the mean difference in infarct size measurement (PET/CT minus histology) was 1.9% (95% confidence interval 0.94% to 2.86%). CONCLUSIONS: In a mice model of permanent coronary occlusion non-invasive measurement of infarct size with high-resolution (18)F-FDG, PET/CT has excellent reproducibility and accuracy. These findings support the use of this methodology in serial studies.
BACKGROUND: This study assessed the reproducibility and accuracy of 2-deoxy-2[(18)F]fluoro-D-glucose ((18)F-FDG) for non-invasive quantification of myocardial infarct size in mice by a high-resolution positron emission tomography (PET)/computed tomography (CT) system. METHODS AND RESULTS:Mice were studied by (18)F-FDG PET/CT 1 week after induction of myocardial infarction by permanent coronary occlusion or sham procedure. In a subset of mice, PET/CT was repeated 2 days apart to assess the reproducibility of infarct size measurements. Histological analysis was used as reference method to validate imaging data. The average difference in infarct size measurements between the first and the second study was -0.42% ± 2.07% (95% confidence interval -2.6 to 1.75) with a repeatability coefficient of 4.05%. At Bland-Altman analysis, the lower and upper limits of agreement between the two repeated studies were -4.46% and 3.63%, respectively, and no correlation between difference and mean was found (P = .89). The concordance correlation coefficient was 0.99 (P < .001) and the intraclass coefficient of correlation was 0.99. A high correlation between PET/CT and histology was found for measurement of infarct size (P < .001). Using Bland-Altman analysis, the mean difference in infarct size measurement (PET/CT minus histology) was 1.9% (95% confidence interval 0.94% to 2.86%). CONCLUSIONS: In a mice model of permanent coronary occlusion non-invasive measurement of infarct size with high-resolution (18)F-FDG, PET/CT has excellent reproducibility and accuracy. These findings support the use of this methodology in serial studies.
Authors: Takashi Kudo; Kazuki Fukuchi; Alexander J Annala; Arion F Chatziioannou; Vivekanand Allada; Magnus Dahlbom; Yuan-Chuan Tai; Masayuki Inubushi; Sung-Cheng Huang; Simon R Cherry; Michael E Phelps; Heinrich R Schelbert Journal: Circulation Date: 2002-07-02 Impact factor: 29.690
Authors: Takahiro Higuchi; Stephan G Nekolla; Antanas Jankaukas; Axel W Weber; Marc C Huisman; Sybille Reder; Sibylle I Ziegler; Markus Schwaiger; Frank M Bengel Journal: J Nucl Med Date: 2007-02 Impact factor: 10.057
Authors: Lars Stegger; Anne-Nadine Hoffmeier; Klaus P Schäfers; Sven Hermann; Otmar Schober; Michael A Schäfers; Gregor Theilmeier Journal: J Nucl Med Date: 2006-11 Impact factor: 10.057
Authors: Zhonglin Liu; George A Kastis; Gail D Stevenson; Harrison H Barrett; Lars R Furenlid; Matthew A Kupinski; Dennis D Patton; Donald W Wilson Journal: J Nucl Med Date: 2002-07 Impact factor: 10.057
Authors: Cinzia Perrino; Gabriele G Schiattarella; Anna Sannino; Gianluigi Pironti; Maria Piera Petretta; Alessandro Cannavo; Giuseppe Gargiulo; Federica Ilardi; Fabio Magliulo; Anna Franzone; Giuseppe Carotenuto; Federica Serino; Giovanna G Altobelli; Vincenzo Cimini; Alberto Cuocolo; Assunta Lombardi; Fernando Goglia; Ciro Indolfi; Bruno Trimarco; Giovanni Esposito Journal: J Am Heart Assoc Date: 2013-05-20 Impact factor: 5.501