Literature DB >> 22359120

Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

H Thomou1, C Sambani, P Kitsiou, G Politis.   

Abstract

We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formation suggests that changes in chromatin are a prerequisite for rosette formation. Rosette formation was most pronounced for 24-h and 48-h cultures. Chromatin decondensation and rosette formation showed identical patterns. The possibility that multilayer rosette formation is directly dependent on conformational changes of chromatin is discussed.

Entities:  

Year:  1988        PMID： 22359120     DOI： 10.1007/BF00145027

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  13 in total

1.  DNA present in multilayer rosette formed by lymphocytes stimulated with phytohaemagglutinin.

Authors:  G Politis; M G Plassara; H Thomou-Politi
Journal:  Nature       Date:  1975-10-09       Impact factor: 49.962

2.  Induction of premature chromosome condensation in CHO cells fused with polyethylene glycol.

Authors:  Y F Lau; R L Brown; F E Arrighi
Journal:  Exp Cell Res       Date:  1977-11       Impact factor: 3.905

3.  Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes.

Authors:  W N Hittelman; P N Rao
Journal:  Exp Cell Res       Date:  1976-07       Impact factor: 3.905

4.  Optimal conditions for the fusion of lymphoid cell lines.

Authors:  J K Stadler; A Ward; E A Adelberg
Journal:  In Vitro       Date:  1975 Jul-Aug

5.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors:  A Böyum
Journal:  Scand J Clin Lab Invest Suppl       Date:  1968

6.  Studies on the human T lymphocyte population. III. Synthesis and release of the lymphocyte receptor for sheep red blood cells by stimulated human T lymphoblasts.

Authors:  F L Owen; M W Fanger
Journal:  J Immunol       Date:  1975-09       Impact factor: 5.422

7.  Induction of premature chromosome condensation at high frequency following polyethylene glycol-mediated fusion of lectin-bound cells.

Authors:  S K Hanks; D B Brown; P N Rao
Journal:  Exp Cell Res       Date:  1982-03       Impact factor: 3.905

8.  Human lymphocyte subpopulations: giant SRBC rosettes.

Authors:  D T Yu
Journal:  J Immunol       Date:  1976-06       Impact factor: 5.422

9.  Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol.

Authors:  R L Davidson; P S Gerald
Journal:  Somatic Cell Genet       Date:  1976-03

10.  A simple method for premature chromosome condensation induction in primary human and rodent cells using polyethylene glycol.

Authors:  G E Pantelias; H D Maillie
Journal:  Somatic Cell Genet       Date:  1983-09
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