Literature DB >> 6623312

A simple method for premature chromosome condensation induction in primary human and rodent cells using polyethylene glycol.

G E Pantelias, H D Maillie.   

Abstract

Even though polyethylene glycol (PEG) has been shown to be a potent fusogen, it has not been widely exploited as an alternative to the Sendai virus for premature chromosome condensation (PCC) induction. A simple, rapid, and reproducible PEG protocol for primary cells in suspension is presented which allows satisfactory cell fusion and PCC indices, giving at the same time high cell viability and low giant, multinucleated cell formation. Technical details for PEG-mediated fusion and premature chromosome condensation induction in human and rat lymphocytes, rodent spleen cells, and spleen and whole body cells of newborn mice are provided. Further applications of the method are suggested.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6623312     DOI： 10.1007/bf01574257

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  17 in total

1.  Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

Authors:  H Thomou; C Sambani; P Kitsiou; G Politis
Journal:  Cytotechnology       Date:  1988-07       Impact factor: 2.058

2.  Nucleolus organizer regions in human lymphocytes as studied with premature chromosome condensation.

Authors:  F Wachtler; C Roubicek; A Schedle; W Mosgöller; G Bretis; H G Schwarzacher
Journal:  Hum Genet       Date:  1990-02       Impact factor: 4.132

3.  Visualizing the dynamics of chromosome structure formation coupled with DNA replication.

Authors:  Eisuke Gotoh
Journal:  Chromosoma       Date:  2007-05-15       Impact factor: 4.316

4.  A cytogenetic methodology to evaluate in vitro the G2-chromosomal radiosensitization induced by chemicals at non-clastogenic doses.

Authors:  V I Hatzi; Georgia I Terzoudi; Vasilios Makropoulos; Gabriel E Pantelias
Journal:  J Genet       Date:  2009-12       Impact factor: 1.166

5.  The mechanism and pattern of banding induced by restriction endonucleases in human chromosomes.

Authors:  M S Bianchi; N O Bianchi; G E Pantelias; S Wolff
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316

6.  Possible target of Abelson virus phosphokinase in cell transformation.

Authors:  G Pantelias; H M Jäck; M Wabl
Journal:  Experientia       Date:  1986-09-15

7.  A cytogenetic investigation of DNA rereplication after hydroxyurea treatment: implications for gene amplification.

Authors:  W F Morgan; J Bodycote; M L Fero; P J Hahn; L N Kapp; G E Pantelias; R B Painter
Journal:  Chromosoma       Date:  1986       Impact factor: 4.316

8.  Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay).

Authors:  Georgia I Terzoudi; Gabriel Pantelias; Firouz Darroudi; Katarzyna Barszczewska; Iwona Buraczewska; Julie Depuydt; Dimka Georgieva; Valeria Hadjidekova; Vasiliki I Hatzi; Ioanna Karachristou; Maria Karakosta; Roberta Meschini; Radhia M'Kacher; Alegria Montoro; Fabrizio Palitti; Antonio Pantelias; Gaetano Pepe; Michelle Ricoul; Laure Sabatier; Natividad Sebastià; Sylwester Sommer; Anne Vral; Demetre Zafiropoulos; Andrzej Wojcik
Journal:  Int J Radiat Biol       Date:  2016-11-04       Impact factor: 2.694

9.  A model of chromosome aberration induction and chronic myeloid leukaemia incidence at low doses.

Authors:  Francesca Ballarini; Andrea Ottolenghi
Journal:  Radiat Environ Biophys       Date:  2004-08-07       Impact factor: 1.925

10.  Refined premature chromosome condensation (G0-PCC) with cryo-preserved mitotic cells for rapid radiation biodosimetry.

Authors:  Usha Yadav; Nagesh N Bhat; Kapil B Shirsaath; Utkarsha S Mungse; Balvinder K Sapra
Journal:  Sci Rep       Date:  2021-06-29       Impact factor: 4.379
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.