Literature DB >> 22358436

Localization of amplified CAD genes on rearranged chromosomes of Chinese hamster cells.

L Ottaggio1, C Agnese, S Bonatti, P Cavolina, A Di Leonardo, M Miele, A Abbondandolo.   

Abstract

Chinese hamster cell lines carrying an amplified CAD region were selected from V79,B7 cells by their resistance to N-phosphonacetyl-L-aspartate (PALA). In one of the selected cell lines, SP PALA (inf1) (supR) L, an acrocentric chromosome with abnormally elongated q arms was identified as a marker for the PALA-resistant phenotype. The marker chromosome carried a homogeneously staining region close to a telomeric nucleolar organizer region. In the same region, localization of amplified CAD sequences was demonstrated by in situ hybridization. The marker chromosome was found to undergo extensive rearrangements. In particular, dicentric chromosomes, occurring with an unusually high incidence, were found to originate from end-fusion of two homologous marker chromosomes.

Entities:  

Year:  1987        PMID： 22358436     DOI： 10.1007/BF00351118

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  12 in total

1.  Overreplication and recombination of DNA in higher eukaryotes: potential consequences and biological implications.

Authors:  R T Schimke; S W Sherwood; A B Hill; R N Johnston
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

2.  Gene amplification causes overproduction of the first three enzymes of UMP synthesis in N-(phosphonacetyl)-L-aspartate-resistant hamster cells.

Authors:  G M Wahl; R A Padgett; G R Stark
Journal:  J Biol Chem       Date:  1979-09-10       Impact factor: 5.157

3.  N-(phosphonacetyl)-L-aspartate, a potent transition state analog inhibitor of aspartate transcarbamylase, blocks proliferation of mammalian cells in culture.

Authors:  E A Swyryd; S S Seaver; G R Stark
Journal:  J Biol Chem       Date:  1974-11-10       Impact factor: 5.157

4.  N-(Phosphonacetyl)-L-aspartate-resistant hamster cells overaccumulate a single mRNA coding for the multifunctional protein that catalyzes the first steps of UMP synthesis.

Authors:  R A Padgett; G M Wahl; P F Coleman; G R Stark
Journal:  J Biol Chem       Date:  1979-02-10       Impact factor: 5.157

5.  Occurrence and evolution of homogeneously staining regions may be due to breakage-fusion-bridge cycles following telomere loss.

Authors:  J K Cowell; O J Miller
Journal:  Chromosoma       Date:  1983       Impact factor: 4.316

6.  General method for cloning amplified DNA by differential screening with genomic probes.

Authors:  O Brison; F Ardeshir; G R Stark
Journal:  Mol Cell Biol       Date:  1982-05       Impact factor: 4.272

7.  Stable mutants of mammalian cells that overproduce the first three enzymes of pyrimidine nucleotide biosynthesis.

Authors:  T D Kempe; E A Swyryd; M Bruist; G R Stark
Journal:  Cell       Date:  1976-12       Impact factor: 41.582

8.  Single-copy and amplified CAD genes in Syrian hamster chromosomes localized by a highly sensitive method for in situ hybridization.

Authors:  G M Wahl; L Vitto; R A Padgett; G R Stark
Journal:  Mol Cell Biol       Date:  1982-03       Impact factor: 4.272

9.  Properties of dispersed, highly repeated DNA within and near the hamster CAD gene.

Authors:  R A Padgett; G M Wahl; G R Stark
Journal:  Mol Cell Biol       Date:  1982-03       Impact factor: 4.272

10.  Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells.

Authors:  G M Wahl; L Vitto; J Rubnitz
Journal:  Mol Cell Biol       Date:  1983-11       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.