Literature DB >> 22337987

Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools.

Wenlan Zhang1, Martina Bielaszewska, Andreas Bauwens, Angelika Fruth, Alexander Mellmann, Helge Karch.   

Abstract

A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

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Year:  2012        PMID： 22337987      PMCID： PMC3347122          DOI： 10.1128/JCM.06817-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

Review 1.  Outbreak of Shiga toxin-producing Escherichia coli (STEC) O104:H4 infection in Germany causes a paradigm shift with regard to human pathogenicity of STEC strains.

Authors:  Lothar Beutin; Annett Martin
Journal:  J Food Prot       Date:  2012-02       Impact factor: 2.077

2.  Shiga toxin activatable by intestinal mucus in Escherichia coli isolated from humans: predictor for a severe clinical outcome.

Authors:  Martina Bielaszewska; Alexander W Friedrich; Thomas Aldick; Robin Schürk-Bulgrin; Helge Karch
Journal:  Clin Infect Dis       Date:  2006-10-02       Impact factor: 9.079

Review 3.  Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome.

Authors:  Phillip I Tarr; Carrie A Gordon; Wayne L Chandler
Journal:  Lancet       Date:  2005 Mar 19-25       Impact factor: 79.321

4.  Pigeons as a possible reservoir of Shiga toxin 2f-producing Escherichia coli pathogenic to humans.

Authors:  Anne-Katharina Sonntag; Elena Zenner; Helge Karch; Martina Bielaszewska
Journal:  Berl Munch Tierarztl Wochenschr       Date:  2005 Nov-Dec       Impact factor: 0.328

5.  Structural and functional differences between disease-associated genes of enterohaemorrhagic Escherichia coli O111.

Authors:  Wenlan Zhang; Alexander Mellmann; Anne-K Sonntag; Lothar Wieler; Martina Bielaszewska; Helmut Tschäpe; Helge Karch; Alexander W Friedrich
Journal:  Int J Med Microbiol       Date:  2006-12-08       Impact factor: 3.473

6.  A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons.

Authors:  H Schmidt; J Scheef; S Morabito; A Caprioli; L H Wieler; H Karch
Journal:  Appl Environ Microbiol       Date:  2000-03       Impact factor: 4.792

7.  Shiga toxin, cytolethal distending toxin, and hemolysin repertoires in clinical Escherichia coli O91 isolates.

Authors:  Martina Bielaszewska; Franziska Stoewe; Angelika Fruth; Wenlan Zhang; Rita Prager; Jens Brockmeyer; Alexander Mellmann; Helge Karch; Alexander W Friedrich
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

8.  Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology.

Authors:  Alexander Mellmann; Dag Harmsen; Craig A Cummings; Emily B Zentz; Shana R Leopold; Alain Rico; Karola Prior; Rafael Szczepanowski; Yongmei Ji; Wenlan Zhang; Stephen F McLaughlin; John K Henkhaus; Benjamin Leopold; Martina Bielaszewska; Rita Prager; Pius M Brzoska; Richard L Moore; Simone Guenther; Jonathan M Rothberg; Helge Karch
Journal:  PLoS One       Date:  2011-07-20       Impact factor: 3.240

9.  Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli.

Authors:  Alexander Mellmann; Martina Bielaszewska; Robin Köck; Alexander W Friedrich; Angelika Fruth; Barbara Middendorf; Dag Harmsen; M Alexander Schmidt; Helge Karch
Journal:  Emerg Infect Dis       Date:  2008-08       Impact factor: 6.883

10.  Sequence-based typing of genetic targets encoded outside of the O-antigen gene cluster is indicative of Shiga toxin-producing Escherichia coli serogroup lineages.

Authors:  Matthew W Gilmour; Adam B Olson; Ashleigh K Andrysiak; Lai-King Ng; Linda Chui
Journal:  J Med Microbiol       Date:  2007-05       Impact factor: 2.472
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  12 in total

1.  Characterization of urinary tract infection-associated Shiga toxin-producing Escherichia coli.

Authors:  Francisco Toval; Roswitha Schiller; Iris Meisen; Johannes Putze; Ivan U Kouzel; Wenlan Zhang; Helge Karch; Martina Bielaszewska; Michael Mormann; Johannes Müthing; Ulrich Dobrindt
Journal:  Infect Immun       Date:  2014-08-25       Impact factor: 3.441

2.  Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.

Authors:  Stefanie A Barth; Rolf Bauerfeind; Christian Berens; Christian Menge
Journal:  Methods Mol Biol       Date:  2021

3.  Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak.

Authors:  Z D Paddock; J Bai; X Shi; D G Renter; T G Nagaraja
Journal:  Appl Environ Microbiol       Date:  2013-03-29       Impact factor: 4.792

4.  Pooled Nucleic Acid Amplification Test for Screening of Stool Specimens for Shiga Toxin-Producing Escherichia coli.

Authors:  Agatha N Jassem; Frank Y Chou; Cathevine Yang; Matthew A Croxen; Katarina D M Pintar; Ana Paccagnella; Linda Hoang; Natalie Prystajecky
Journal:  J Clin Microbiol       Date:  2016-08-24       Impact factor: 5.948

5.  Specific detection of enteroaggregative hemorrhagic Escherichia coli O104:H4 strains by use of the CRISPR locus as a target for a diagnostic real-time PCR.

Authors:  Sabine Delannoy; Lothar Beutin; Ylanna Burgos; Patrick Fach
Journal:  J Clin Microbiol       Date:  2012-08-15       Impact factor: 5.948

6.  Novel Multiplex PCR Method and Genome Sequence-Based Analog for High-Resolution Subclonal Assignment and Characterization of Escherichia coli Sequence Type 131 Isolates.

Authors:  Brian D Johnston; David M Gordon; Samantha Burn; Timothy J Johnson; Bonnie P Weber; Elizabeth A Miller; James R Johnson
Journal:  Microbiol Spectr       Date:  2022-05-23

7.  Use of clustered regularly interspaced short palindromic repeat sequence polymorphisms for specific detection of enterohemorrhagic Escherichia coli strains of serotypes O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7 by real-time PCR.

Authors:  Sabine Delannoy; Lothar Beutin; Patrick Fach
Journal:  J Clin Microbiol       Date:  2012-10-03       Impact factor: 5.948

8.  Lability of the pAA Virulence Plasmid in Escherichia coli O104:H4: Implications for Virulence in Humans.

Authors:  Wenlan Zhang; Martina Bielaszewska; Lisa Kunsmann; Alexander Mellmann; Andreas Bauwens; Robin Köck; Annelene Kossow; Agnes Anders; Sören Gatermann; Helge Karch
Journal:  PLoS One       Date:  2013-06-21       Impact factor: 3.240

9.  Comprehensive Characterization of Escherichia coli O104:H4 Isolated from Patients in the Netherlands.

Authors:  Mithila Ferdous; Kai Zhou; Richard F de Boer; Alexander W Friedrich; Anna M D Kooistra-Smid; John W A Rossen
Journal:  Front Microbiol       Date:  2015-12-02       Impact factor: 5.640

Review 10.  Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coli.

Authors:  Brendon D Parsons; Nathan Zelyas; Byron M Berenger; Linda Chui
Journal:  Front Microbiol       Date:  2016-04-12       Impact factor: 5.640
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