Literature DB >> 22194286

Simple cloning via direct transformation of PCR product (DNA Multimer) to Escherichia coli and Bacillus subtilis.

Chun You1, Xiao-Zhou Zhang, Y-H Percival Zhang.   

Abstract

We developed a general restriction enzyme-free and ligase-free method for subcloning up to three DNA fragments into any location of a plasmid. The DNA multimer generated by prolonged overlap extension PCR was directly transformed in Escherichia coli [e.g., TOP10, DH5α, JM109, and BL21(DE3)] and Bacillus subtilis for obtaining chimeric plasmids.

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Year:  2011        PMID： 22194286      PMCID： PMC3294473          DOI： 10.1128/AEM.07105-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

Authors:  A J Walhout; G F Temple; M A Brasch; J L Hartley; M A Lorson; S van den Heuvel; M Vidal
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

2.  One-step random mutagenesis by error-prone rolling circle amplification.

Authors:  Ryota Fujii; Motomitsu Kitaoka; Kiyoshi Hayashi
Journal:  Nucleic Acids Res       Date:  2004-10-26       Impact factor: 16.971

3.  In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations.

Authors:  Baogong Zhu; Guifang Cai; Emily O Hall; Gordon J Freeman
Journal:  Biotechniques       Date:  2007-09       Impact factor: 1.993

4.  Specificity and affinity of substrate binding by a family 17 carbohydrate-binding module from Clostridium cellulovorans cellulase 5A.

Authors:  A B Boraston; P Chiu; R A Warren; D G Kilburn
Journal:  Biochemistry       Date:  2000-09-12       Impact factor: 3.162

5.  Ligation independent cloning irrespective of restriction site compatibility.

Authors:  C Li; R M Evans
Journal:  Nucleic Acids Res       Date:  1997-10-15       Impact factor: 16.971

6.  Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

Authors:  Anton V Bryksin; Ichiro Matsumura
Journal:  Biotechniques       Date:  2010-06       Impact factor: 1.993

7.  A novel simple and rapid PCR-based site-directed mutagenesis method.

Authors:  Imen Rabhi; Naouel Guedel; Imen Chouk; Khaled Zerria; M Ridha Barbouche; Koussay Dellagi; Dahmani M Fathallah
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

8.  A new logic for DNA engineering using recombination in Escherichia coli.

Authors:  Y Zhang; F Buchholz; J P Muyrers; A F Stewart
Journal:  Nat Genet       Date:  1998-10       Impact factor: 38.330

9.  Cloning of cDNA encoding a novel mouse DNA topoisomerase III (Topo IIIbeta) possessing negatively supercoiled DNA relaxing activity, whose message is highly expressed in the testis.

Authors:  T Seki; M Seki; R Onodera; T Katada; T Enomoto
Journal:  J Biol Chem       Date:  1998-10-30       Impact factor: 5.157

10.  One-step DNA fragment assembly and circularization for gene cloning.

Authors:  Peijun Zuo; Bakr M Rabie
Journal:  Curr Issues Mol Biol       Date:  2009-06-03       Impact factor: 2.081
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  37 in total

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Authors:  Zhihong Huang; Ao Li; Mengjia Pan; Wenbi Wu; Meijin Yuan; Kai Yang
Journal:  Virol Sin       Date:  2015-10-19       Impact factor: 4.327

2.  A spectrophotometric method for high-throughput screening of α-l-rhamnosidase activity on rutin coupled with a β-d-glucosidase assay.

Authors:  Bin-Chun Li; Bo Peng; Tian Zhang; Yan-Qin Li; Guo-Bin Ding
Journal:  3 Biotech       Date:  2019-05-21       Impact factor: 2.406

3.  An ATP-free in vitro synthetic enzymatic biosystem facilitating one-pot stoichiometric conversion of starch to mannitol.

Authors:  Xinlei Wei; Qiangzi Li; Congcong Hu; Chun You
Journal:  Appl Microbiol Biotechnol       Date:  2021-02-05       Impact factor: 4.813

4.  Coevolution of both Thermostability and Activity of Polyphosphate Glucokinase from Thermobifida fusca YX.

Authors:  Wei Zhou; Rui Huang; Zhiguang Zhu; Yi-Heng P Job Zhang
Journal:  Appl Environ Microbiol       Date:  2018-08-01       Impact factor: 4.792

5.  Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima.

Authors:  Julia S Martín del Campo; You Chun; Jae-Eung Kim; Rodrigo Patiño; Y-H Percival Zhang
Journal:  J Ind Microbiol Biotechnol       Date:  2013-04-14       Impact factor: 3.346

6.  High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis.

Authors:  Jingqi Chen; Yueming Zhu; Gang Fu; Yafeng Song; Zhaoxia Jin; Yuanxia Sun; Dawei Zhang
Journal:  J Ind Microbiol Biotechnol       Date:  2016-08-20       Impact factor: 3.346

7.  A YadA-like autotransporter, Hag1 in Veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, Porphyromonas gingivalis, and human oral buccal cells.

Authors:  Peng Zhou; Jinman Liu; Justin Merritt; Fengxia Qi
Journal:  Mol Oral Microbiol       Date:  2015-01-19       Impact factor: 3.563

8.  Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells.

Authors:  Frederic Laurent; Sophie Trouillet-Assant; Yousef Maali; Alan Diot; Patrícia Martins-Simões; Michele Bes; Daniel Bouvard; François Vandenesch; Paul O Verhoeven
Journal:  Infect Immun       Date:  2020-04-20       Impact factor: 3.441

9.  Cloning-independent plasmid construction for genetic studies in streptococci.

Authors:  Zhoujie Xie; Fengxia Qi; Justin Merritt
Journal:  J Microbiol Methods       Date:  2013-05-12       Impact factor: 2.363

10.  Unchaining miniBacillus Strain PG10: Relief of FlgM-Mediated Repression of Autolysin Genes.

Authors:  Amanda Y van Tilburg; Julius A Fülleborn; Alexander Reder; Uwe Völker; Jörg Stülke; Auke J van Heel; Oscar P Kuipers
Journal:  Appl Environ Microbiol       Date:  2021-08-26       Impact factor: 4.792
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