Literature DB >> 22155214

Immobilization of tyrosinase on chitosan-clay composite beads.

Ayşe Dinçer1, Seda Becerik, Tülin Aydemir.   

Abstract

Tyrosinase was immobilized on glutaraldehyde crosslinked chitosan-clay composite beads and used for phenol removal. Immobilization yield, loading efficiency and activity of tyrosinase immobilized beads were found as 67%, 25% and 1400 U/g beads respectively. Optimum pH of the free and immobilized enzyme was found as pH 7.0. Optimum temperature of the free and immobilized enzyme was determined as 25-30 °C and 25 °C respectively. The kinetic parameters of free and immobilized tyrosinase were calculated using l-catechol as a substrate and K(m) value for free and immobilized tyrosinase were found as 0.93 mM and 1.7 mM respectively. After seven times of repeated tests, each over 150 min, the efficiency of phenol removal using same immobilized tyrosinase beads were decreased to 43%.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID： 22155214     DOI： 10.1016/j.ijbiomac.2011.11.020

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN： 0141-8130            Impact factor:   6.953


  8 in total

1.  Fabrication of the robust and recyclable tyrosinase-harboring biocatalyst using ethylenediamine functionalized superparamagnetic nanoparticles: nanocarrier characterization and immobilized enzyme properties.

Authors:  Kourosh Abdollahi; Farshad Yazdani; Reza Panahi
Journal:  J Biol Inorg Chem       Date:  2019-07-29       Impact factor: 3.358

Review 2.  Immobilized enzymes and cell systems: an approach to the removal of phenol and the challenges to incorporate nanoparticle-based technology.

Authors:  Genesis Escobedo-Morales; Javier Ulises Hernández-Beltrán; Ayerim Yedid Hernández-Almanza; Miriam Paulina Luévanos-Escareño
Journal:  World J Microbiol Biotechnol       Date:  2022-01-19       Impact factor: 3.312

3.  Functionalized Tyrosinase-Lignin Nanoparticles as Sustainable Catalysts for the Oxidation of Phenols.

Authors:  Eliana Capecchi; Davide Piccinino; Ines Delfino; Paolo Bollella; Riccarda Antiochia; Raffaele Saladino
Journal:  Nanomaterials (Basel)       Date:  2018-06-15       Impact factor: 5.076

4.  Modifying Thermostability and Reusability of Hyperthermophilic Mannanase by Immobilization on Glutaraldehyde Cross-Linked Chitosan Beads.

Authors:  Beenish Sadaqat; Chong Sha; Mudasir Ahmad Dar; Maruti J Dhanavade; Kailas D Sonawane; Hassan Mohamed; Weilan Shao; Yuanda Song
Journal:  Biomolecules       Date:  2022-07-18

5.  Immobilization of Amano lipase AK from Pseudomonas fluorescens on different types of chitosan-containing supports: use in the kinetic resolution of rac-indanol.

Authors:  Thiago de Sousa Fonseca; Ulisses Marcondes Freire de Oliveira; Maria da Conceição Ferreira de Oliveira; Telma Leda Gomes de Lemos; Marcos Reinaldo da Silva; Nathalia Saraiva Rios; Luciana Rocha Barros Gonçalves; Marcos Carlos de Mattos
Journal:  Bioprocess Biosyst Eng       Date:  2021-01-03       Impact factor: 3.210

Review 6.  Engineered tyrosinases with broadened bio-catalysis scope: immobilization using nanocarriers and applications.

Authors:  Asim Hussain; Hamza Rafeeq; Muhammad Qasim; Zara Jabeen; Muhammad Bilal; Marcelo Franco; Hafiz M N Iqbal
Journal:  3 Biotech       Date:  2021-07-05       Impact factor: 2.893

7.  Immobilization of Trypsin from Porcine Pancreas onto Chitosan Nonwoven by Covalent Bonding.

Authors:  Jung Soo Kim; Sohee Lee
Journal:  Polymers (Basel)       Date:  2019-09-06       Impact factor: 4.329

Review 8.  Enzyme-polymeric/inorganic metal oxide/hybrid nanoparticle bio-conjugates in the development of therapeutic and biosensing platforms.

Authors:  Suliman Khan; Mohammad Mahdi Nejadi Babadaei; Anwarul Hasan; Zehra Edis; Farnoosh Attar; Rabeea Siddique; Qian Bai; Majid Sharifi; Mojtaba Falahati
Journal:  J Adv Res       Date:  2021-02-04       Impact factor: 10.479
  8 in total

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