Immobilized enzymes and cell systems: an approach to the removal of phenol and the challenges to incorporate nanoparticle-based technology.

The presence of phenol in wastewater poses a risk to ecosystems and human health. The traditional processes to remove phenol from wastewater, although effective, have several drawbacks. The best alternative is the application of ecological biotechnology tools since they involve biological systems (enzymes and microorganisms) with moderate economic and environmental impact. However, these systems have a high sensitivity to environmental factors and high substrate concentrations that reduce their effectiveness in phenol removal. This can be overcome by immobilization-based technology to increase the performance of enzymes and bacteria. A key component to ensure successful immobilization is the material (polymeric matrices) used as support for the biological system. In addition, by incorporating magnetic nanoparticles into conventional immobilized systems, a low-cost process is achieved but, most importantly, the magnetically immobilized system can be recovered, recycled, and reused. In this review, we study the existing alternatives for treating wastewater with phenol, from physical and chemical to biological techniques. The latter focus on the immobilization of enzymes and microorganisms. The characteristics of the support materials that ensure the viability of the immobilization are compared. In addition, the challenges and opportunities that arise from incorporating magnetic nanoparticles in immobilized systems are addressed.