Literature DB >> 22138822

Controlled gene expression in primary Lgr5 organoid cultures.

Bon-Kyoung Koo1, Daniel E Stange, Toshiro Sato, Wouter Karthaus, Henner F Farin, Meritxell Huch, Johan H van Es, Hans Clevers.   

Abstract

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

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Year:  2011        PMID： 22138822     DOI： 10.1038/nmeth.1802

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  14 in total

1.  Initial sequencing and comparative analysis of the mouse genome.

Authors:  Robert H Waterston; Kerstin Lindblad-Toh; Ewan Birney; Jane Rogers; Josep F Abril; Pankaj Agarwal; Richa Agarwala; Rachel Ainscough; Marina Alexandersson; Peter An; Stylianos E Antonarakis; John Attwood; Robert Baertsch; Jonathon Bailey; Karen Barlow; Stephan Beck; Eric Berry; Bruce Birren; Toby Bloom; Peer Bork; Marc Botcherby; Nicolas Bray; Michael R Brent; Daniel G Brown; Stephen D Brown; Carol Bult; John Burton; Jonathan Butler; Robert D Campbell; Piero Carninci; Simon Cawley; Francesca Chiaromonte; Asif T Chinwalla; Deanna M Church; Michele Clamp; Christopher Clee; Francis S Collins; Lisa L Cook; Richard R Copley; Alan Coulson; Olivier Couronne; James Cuff; Val Curwen; Tim Cutts; Mark Daly; Robert David; Joy Davies; Kimberly D Delehaunty; Justin Deri; Emmanouil T Dermitzakis; Colin Dewey; Nicholas J Dickens; Mark Diekhans; Sheila Dodge; Inna Dubchak; Diane M Dunn; Sean R Eddy; Laura Elnitski; Richard D Emes; Pallavi Eswara; Eduardo Eyras; Adam Felsenfeld; Ginger A Fewell; Paul Flicek; Karen Foley; Wayne N Frankel; Lucinda A Fulton; Robert S Fulton; Terrence S Furey; Diane Gage; Richard A Gibbs; Gustavo Glusman; Sante Gnerre; Nick Goldman; Leo Goodstadt; Darren Grafham; Tina A Graves; Eric D Green; Simon Gregory; Roderic Guigó; Mark Guyer; Ross C Hardison; David Haussler; Yoshihide Hayashizaki; LaDeana W Hillier; Angela Hinrichs; Wratko Hlavina; Timothy Holzer; Fan Hsu; Axin Hua; Tim Hubbard; Adrienne Hunt; Ian Jackson; David B Jaffe; L Steven Johnson; Matthew Jones; Thomas A Jones; Ann Joy; Michael Kamal; Elinor K Karlsson; Donna Karolchik; Arkadiusz Kasprzyk; Jun Kawai; Evan Keibler; Cristyn Kells; W James Kent; Andrew Kirby; Diana L Kolbe; Ian Korf; Raju S Kucherlapati; Edward J Kulbokas; David Kulp; Tom Landers; J P Leger; Steven Leonard; Ivica Letunic; Rosie Levine; Jia Li; Ming Li; Christine Lloyd; Susan Lucas; Bin Ma; Donna R Maglott; Elaine R Mardis; Lucy Matthews; Evan Mauceli; John H Mayer; Megan McCarthy; W Richard McCombie; Stuart McLaren; Kirsten McLay; John D McPherson; Jim Meldrim; Beverley Meredith; Jill P Mesirov; Webb Miller; Tracie L Miner; Emmanuel Mongin; Kate T Montgomery; Michael Morgan; Richard Mott; James C Mullikin; Donna M Muzny; William E Nash; Joanne O Nelson; Michael N Nhan; Robert Nicol; Zemin Ning; Chad Nusbaum; Michael J O'Connor; Yasushi Okazaki; Karen Oliver; Emma Overton-Larty; Lior Pachter; Genís Parra; Kymberlie H Pepin; Jane Peterson; Pavel Pevzner; Robert Plumb; Craig S Pohl; Alex Poliakov; Tracy C Ponce; Chris P Ponting; Simon Potter; Michael Quail; Alexandre Reymond; Bruce A Roe; Krishna M Roskin; Edward M Rubin; Alistair G Rust; Ralph Santos; Victor Sapojnikov; Brian Schultz; Jörg Schultz; Matthias S Schwartz; Scott Schwartz; Carol Scott; Steven Seaman; Steve Searle; Ted Sharpe; Andrew Sheridan; Ratna Shownkeen; Sarah Sims; Jonathan B Singer; Guy Slater; Arian Smit; Douglas R Smith; Brian Spencer; Arne Stabenau; Nicole Stange-Thomann; Charles Sugnet; Mikita Suyama; Glenn Tesler; Johanna Thompson; David Torrents; Evanne Trevaskis; John Tromp; Catherine Ucla; Abel Ureta-Vidal; Jade P Vinson; Andrew C Von Niederhausern; Claire M Wade; Melanie Wall; Ryan J Weber; Robert B Weiss; Michael C Wendl; Anthony P West; Kris Wetterstrand; Raymond Wheeler; Simon Whelan; Jamey Wierzbowski; David Willey; Sophie Williams; Richard K Wilson; Eitan Winter; Kim C Worley; Dudley Wyman; Shan Yang; Shiaw-Pyng Yang; Evgeny M Zdobnov; Michael C Zody; Eric S Lander
Journal:  Nature       Date:  2002-12-05       Impact factor: 49.962

2.  Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells.

Authors:  Johan H van Es; Marielle E van Gijn; Orbicia Riccio; Maaike van den Born; Marc Vooijs; Harry Begthel; Miranda Cozijnsen; Sylvie Robine; Doug J Winton; Freddy Radtke; Hans Clevers
Journal:  Nature       Date:  2005-06-16       Impact factor: 49.962

3.  Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.

Authors:  Toshiro Sato; Daniel E Stange; Marc Ferrante; Robert G J Vries; Johan H Van Es; Stieneke Van den Brink; Winan J Van Houdt; Apollo Pronk; Joost Van Gorp; Peter D Siersema; Hans Clevers
Journal:  Gastroenterology       Date:  2011-09-02       Impact factor: 22.682

4.  Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling.

Authors:  Wim de Lau; Nick Barker; Teck Y Low; Bon-Kyoung Koo; Vivian S W Li; Hans Teunissen; Pekka Kujala; Andrea Haegebarth; Peter J Peters; Marc van de Wetering; Daniel E Stange; Johan E van Es; Daniele Guardavaccaro; Richard B M Schasfoort; Yasuaki Mohri; Katsuhiko Nishimori; Shabaz Mohammed; Albert J R Heck; Hans Clevers
Journal:  Nature       Date:  2011-07-04       Impact factor: 49.962

Review 5.  Current issues in mouse genome engineering.

Authors:  Stefan Glaser; Konstantinos Anastassiadis; A Francis Stewart
Journal:  Nat Genet       Date:  2005-11       Impact factor: 38.330

6.  Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts.

Authors:  Toshiro Sato; Johan H van Es; Hugo J Snippert; Daniel E Stange; Robert G Vries; Maaike van den Born; Nick Barker; Noah F Shroyer; Marc van de Wetering; Hans Clevers
Journal:  Nature       Date:  2010-11-28       Impact factor: 49.962

7.  Identification of stem cells in small intestine and colon by marker gene Lgr5.

Authors:  Nick Barker; Johan H van Es; Jeroen Kuipers; Pekka Kujala; Maaike van den Born; Miranda Cozijnsen; Andrea Haegebarth; Jeroen Korving; Harry Begthel; Peter J Peters; Hans Clevers
Journal:  Nature       Date:  2007-10-14       Impact factor: 49.962

8.  Growth suppression of pre-T acute lymphoblastic leukemia cells by inhibition of notch signaling.

Authors:  Andrew P Weng; Yunsun Nam; Michael S Wolfe; Warren S Pear; James D Griffin; Stephen C Blacklow; Jon C Aster
Journal:  Mol Cell Biol       Date:  2003-01       Impact factor: 4.272

9.  Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155.

Authors:  Kwan-Ho Chung; Christopher C Hart; Sarmad Al-Bassam; Adam Avery; Jennifer Taylor; Paresh D Patel; Anne B Vojtek; David L Turner
Journal:  Nucleic Acids Res       Date:  2006-04-13       Impact factor: 16.971

10.  Improved coinfection with amphotropic pseudotyped retroviral vectors.

Authors:  Yuehong Wu; David W Melton; Yong Zhang; Peter J Hornsby
Journal:  J Biomed Biotechnol       Date:  2009-05-25
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  133 in total

1.  Efficient genetic engineering of human intestinal organoids using electroporation.

Authors:  Masayuki Fujii; Mami Matano; Kosaku Nanki; Toshiro Sato
Journal:  Nat Protoc       Date:  2015-09-03       Impact factor: 13.491

2.  Retroviral Transduction of T Cells and T Cell Precursors.

Authors:  Amie Simmons; José Alberola-Ila
Journal:  Methods Mol Biol       Date:  2016

3.  Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning.

Authors:  Lingling Xian; Lionel Chia; Dan Georgess; Li Luo; Shuai Shuai; Andrew J Ewald; Linda M S Resar
Journal:  J Vis Exp       Date:  2019-05-10       Impact factor: 1.355

Review 4.  Update on small intestinal stem cells.

Authors:  Valentina Tesori; Maria Ausiliatrice Puglisi; Wanda Lattanzi; Giovanni Battista Gasbarrini; Antonio Gasbarrini
Journal:  World J Gastroenterol       Date:  2013-08-07       Impact factor: 5.742

5.  General Strategy for Direct Cytosolic Protein Delivery via Protein-Nanoparticle Co-engineering.

Authors:  Rubul Mout; Moumita Ray; Tristan Tay; Kanae Sasaki; Gulen Yesilbag Tonga; Vincent M Rotello
Journal:  ACS Nano       Date:  2017-06-15       Impact factor: 15.881

6.  Gene manipulation in liver ductal organoids by optimized recombinant adeno-associated virus vectors.

Authors:  Jinsong Wei; Gai Ran; Xin Wang; Ning Jiang; Jianqing Liang; Xinhua Lin; Chen Ling; Bing Zhao
Journal:  J Biol Chem       Date:  2019-07-31       Impact factor: 5.157

7.  A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression.

Authors:  Arianna Fumagalli; Saskia J E Suijkerbuijk; Harry Begthel; Evelyne Beerling; Koen C Oost; Hugo J Snippert; Jacco van Rheenen; Jarno Drost
Journal:  Nat Protoc       Date:  2018-01-04       Impact factor: 13.491

8.  A 3-D enteroid-based model to study T-cell and epithelial cell interaction.

Authors:  Aneta Rogoz; Bernardo S Reis; Roos A Karssemeijer; Daniel Mucida
Journal:  J Immunol Methods       Date:  2015-04-02       Impact factor: 2.303

Review 9.  Stem cell-derived organoids and their application for medical research and patient treatment.

Authors:  Sina Bartfeld; Hans Clevers
Journal:  J Mol Med (Berl)       Date:  2017-04-08       Impact factor: 4.599

10.  Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori.

Authors:  Sina Bartfeld; Hans Clevers
Journal:  J Vis Exp       Date:  2015-11-12       Impact factor: 1.355
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