Literature DB >> 22108495

Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.

Leisha J Richardson1, Mirjam Kaestli, Mark Mayo, Jolene R Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M Wagner, Paul S Keim, Bart J Currie.   

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID: 22108495      PMCID: PMC3249147          DOI: 10.1016/j.mimet.2011.10.023

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  13 in total

1.  Purification and characterization of PCR-inhibitory components in blood cells.

Authors:  W A Al-Soud; P Rådström
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

2.  Sensitive and specific molecular detection of Burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern Australia.

Authors:  Mirjam Kaestli; Mark Mayo; Glenda Harrington; Felicity Watt; Jason Hill; Daniel Gal; Bart J Currie
Journal:  Appl Environ Microbiol       Date:  2007-09-14       Impact factor: 4.792

3.  Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences.

Authors:  Pascale Bourhy; Sylvie Bremont; Farida Zinini; Claude Giry; Mathieu Picardeau
Journal:  J Clin Microbiol       Date:  2011-04-06       Impact factor: 5.948

4.  Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.

Authors:  Ella M Meumann; Ryan T Novak; Daniel Gal; Mirjam E Kaestli; Mark Mayo; Joshua P Hanson; Emma Spencer; Mindy B Glass; Jay E Gee; Patricia P Wilkins; Bart J Currie
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

5.  Identification of Pseudomonas pseudomallei in clinical practice: use of simple screening tests and API 20NE.

Authors:  D A Dance; V Wuthiekanun; P Naigowit; N J White
Journal:  J Clin Pathol       Date:  1989-06       Impact factor: 3.411

6.  Prognostic significance of quantitative bacteremia in septicemic melioidosis.

Authors:  A L Walsh; M D Smith; V Wuthiekanun; Y Suputtamongkol; W Chaowagul; D A Dance; B Angus; N J White
Journal:  Clin Infect Dis       Date:  1995-12       Impact factor: 9.079

7.  The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study.

Authors:  Bart J Currie; Linda Ward; Allen C Cheng
Journal:  PLoS Negl Trop Dis       Date:  2010-11-30

8.  Speed of detection of Burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome.

Authors:  C Tiangpitayakorn; S Songsivilai; N Piyasangthong; T Dharakul
Journal:  Am J Trop Med Hyg       Date:  1997-07       Impact factor: 2.345

9.  Circulating bacterial-derived DNA fragments and markers of inflammation in chronic hemodialysis patients.

Authors:  Maurizio Bossola; Maurizio Sanguinetti; Donata Scribano; Cecilia Zuppi; Stefania Giungi; Giovanna Luciani; Riccardo Torelli; Brunella Posteraro; Giovanni Fadda; Luigi Tazza
Journal:  Clin J Am Soc Nephrol       Date:  2008-12-31       Impact factor: 8.237

10.  BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

Authors:  Jolene R Bowers; David M Engelthaler; Jennifer L Ginther; Talima Pearson; Sharon J Peacock; Apichai Tuanyok; David M Wagner; Bart J Currie; Paul S Keim
Journal:  PLoS One       Date:  2010-11-12       Impact factor: 3.240

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  12 in total

1.  Rapid Filter-Based Detection and Culture of Burkholderia pseudomallei from Small Volumes of Urine.

Authors:  Pierre A Michel; Christine Lascols; Jay E Gee; Linda M Weigel; David Sue
Journal:  J Clin Microbiol       Date:  2017-06-21       Impact factor: 5.948

2.  Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis.

Authors:  Ornuma Sangwichian; Toni Whistler; Arnone Nithichanon; Chidchamai Kewcharoenwong; Myint Myint Sein; Chawitar Arayanuphum; Narisara Chantratita; Ganjana Lertmemongkolchai
Journal:  J Clin Microbiol       Date:  2020-06-24       Impact factor: 5.948

Review 3.  Human Melioidosis.

Authors:  I Gassiep; M Armstrong; R Norton
Journal:  Clin Microbiol Rev       Date:  2020-03-11       Impact factor: 26.132

4.  Comparison of TaqMan PCR assays for detection of the melioidosis agent Burkholderia pseudomallei in clinical specimens.

Authors:  Mirjam Kaestli; Leisha J Richardson; Rebecca E Colman; Apichai Tuanyok; Erin P Price; Jolene R Bowers; Mark Mayo; Erin Kelley; Meagan L Seymour; Derek S Sarovich; Talima Pearson; David M Engelthaler; David M Wagner; Paul S Keim; James M Schupp; Bart J Currie
Journal:  J Clin Microbiol       Date:  2012-03-21       Impact factor: 5.948

5.  Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay.

Authors:  Gemma Robertson; Alanna Sorenson; Brenda Govan; Natkunam Ketheesan; Raymond Houghton; Hongjing Chen; David AuCoin; Michael Dillon; Robert Norton
Journal:  J Med Microbiol       Date:  2015-06-07       Impact factor: 2.472

Review 6.  Melioidosis.

Authors:  W Joost Wiersinga; Harjeet S Virk; Alfredo G Torres; Bart J Currie; Sharon J Peacock; David A B Dance; Direk Limmathurotsakul
Journal:  Nat Rev Dis Primers       Date:  2018-02-01       Impact factor: 52.329

Review 7.  Burkholderia Pseudomallei Causing Bone and Joint Infections: A Clinical Update.

Authors:  Nadeem Sajjad Raja; Christine Scarsbrook
Journal:  Infect Dis Ther       Date:  2016-01-04

8.  Clinical definitions of melioidosis.

Authors:  Allen C Cheng; Bart J Currie; David A B Dance; Simon G P Funnell; Direk Limmathurotsakul; Andrew J H Simpson; Sharon J Peacock
Journal:  Am J Trop Med Hyg       Date:  2013-03       Impact factor: 2.345

9.  Comparison of DNA extraction kits for detection of Burkholderia pseudomallei in spiked human whole blood using real-time PCR.

Authors:  Nicole L Podnecky; Mindy G Elrod; Bruce R Newton; Leslie A Dauphin; Jianrong Shi; Sutthinan Chawalchitiporn; Henry C Baggett; Alex R Hoffmaster; Jay E Gee
Journal:  PLoS One       Date:  2013-02-27       Impact factor: 3.240

10.  Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis.

Authors:  Raymond L Houghton; Dana E Reed; Mark A Hubbard; Michael J Dillon; Hongjing Chen; Bart J Currie; Mark Mayo; Derek S Sarovich; Vanessa Theobald; Direk Limmathurotsakul; Gumphol Wongsuvan; Narisara Chantratita; Sharon J Peacock; Alex R Hoffmaster; Brea Duval; Paul J Brett; Mary N Burtnick; David P Aucoin
Journal:  PLoS Negl Trop Dis       Date:  2014-03-20
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