| Literature DB >> 26055557 |
Gemma Robertson1, Alanna Sorenson2, Brenda Govan2, Natkunam Ketheesan2, Raymond Houghton3, Hongjing Chen3, David AuCoin4, Michael Dillon4, Robert Norton1.
Abstract
The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.Entities:
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Year: 2015 PMID： 26055557 PMCID： PMC5972306 DOI： 10.1099/jmm.0.000098
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472