| Literature DB >> 22094804 |
Abstract
The cDNA display method is a robust in vitro display technology that converts an unstable mRNA-protein fusion (mRNA display) to a stable mRNA/cDNA-protein fusion (cDNA display) whose cDNA is covalently linked to its encoded protein using a well-designed puromycin linker. We provide technical details for preparing cDNA display molecules and for the synthesis of the puromycin linker for the purpose of screening the functional proteins and peptides.Entities:
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Year: 2012 PMID： 22094804 DOI： 10.1007/978-1-61779-379-0_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745