| Literature DB >> 22078557 |
Sangmi Jun1, Danxia Ke, Karl Debiec, Gongpu Zhao, Xin Meng, Zandrea Ambrose, Gregory A Gibson, Simon C Watkins, Peijun Zhang.
Abstract
Cryo-electron tomography (cryoET) allows 3D visualization of cellular structures at molecular resolution in a close-to-native state and therefore has the potential to help elucidate early events of HIV-1 infection in host cells. However, structural details of infecting HIV-1 have not been observed, due to technological challenges in working with rare and dynamic HIV-1 particles in human cells. Here, we report structural analysis of HIV-1 and host-cell interactions by means of a correlative high-speed 3D live-cell-imaging and cryoET method. Using this method, we showed under near-native conditions that intact hyperstable mutant HIV-1 cores are released into the cytoplasm of host cells. We further obtained direct evidence to suggest that a hyperstable mutant capsid, E45A, showed delayed capsid disassembly compared to the wild-type capsid. Together, these results demonstrate the advantages of our correlative live-cell and cryoET approach for imaging dynamic processes, such as viral infection. Copyright ÂEntities:
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Year: 2011 PMID： 22078557 PMCID： PMC3217200 DOI： 10.1016/j.str.2011.09.006
Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006