Literature DB >> 31396855

Advances in Cryo-Correlative Light and Electron Microscopy: Applications for Studying Molecular and Cellular Events.

Sangmi Jun1,2,3, Hyun-Joo Ro4,5,6, Anahita Bharda7, Seung Il Kim4,5,6, Dooil Jeoung7, Hyun Suk Jung8.   

Abstract

Cryo-correlative light and electron microscopy (Cryo-CLEM) is materializing as a widespread approach amalgamating the advantages of both fluorescence light microscopy (FLM) as well as three dimensional (3D) cryo-electron tomography (cryo-ET) to reveal the ultrastructure of significant target molecules with specific cellular functions. Cryo-CLEM allows imaging of cells by means of fluorescence microscopy exhibiting the location of the destined molecule at high temporal and spatial resolution while cryo-ET is employed to analyze the 3D structure at a molecular resolution in close-to-physiological condition. Present review focuses upon the practical strategies for Cryo-CLEM and recent technical developments that will assist the broad implementation of this technique to investigate and answer questions pertaining to various biological events occurring in the cell.

Keywords:  3D structure; Cryo-CLEM; Cryo-FLM; Cryo-stage; Live-cell imaging

Mesh：

Substances：

Year:  2019        PMID： 31396855     DOI： 10.1007/s10930-019-09856-1

Source DB:  PubMed          Journal:  Protein J        ISSN： 1572-3887            Impact factor:   2.371


  50 in total

1.  Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography.

Authors:  Vladan Lucić; Albrecht H Kossel; Ting Yang; Tobias Bonhoeffer; Wolfgang Baumeister; Anna Sartori
Journal:  J Struct Biol       Date:  2007-08-29       Impact factor: 2.867

Review 2.  Correlative cryo-light microscopy and cryo-electron tomography: from cellular territories to molecular landscapes.

Authors:  Jürgen M Plitzko; Alexander Rigort; Andrew Leis
Journal:  Curr Opin Biotechnol       Date:  2009-04-01       Impact factor: 9.740

3.  Correlative light and electron microscopy: from live cell dynamic to 3D ultrastructure.

Authors:  Coralie Spiegelhalter; Jocelyn F Laporte; Yannick Schwab
Journal:  Methods Mol Biol       Date:  2014

4.  Correlative microscopy for 3D structural analysis of dynamic interactions.

Authors:  Sangmi Jun; Gongpu Zhao; Jiying Ning; Gregory A Gibson; Simon C Watkins; Peijun Zhang
Journal:  J Vis Exp       Date:  2013-06-24       Impact factor: 1.355

Review 5.  Correlated light and electron microscopy: ultrastructure lights up!

Authors:  Pascal de Boer; Jacob P Hoogenboom; Ben N G Giepmans
Journal:  Nat Methods       Date:  2015-06       Impact factor: 28.547

6.  Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision.

Authors:  Wanda Kukulski; Martin Schorb; Sonja Welsch; Andrea Picco; Marko Kaksonen; John A G Briggs
Journal:  J Cell Biol       Date:  2011-01-03       Impact factor: 10.539

7.  Structural variability and complexity of the giant Pithovirus sibericum particle revealed by high-voltage electron cryo-tomography and energy-filtered electron cryo-microscopy.

Authors:  Kenta Okamoto; Naoyuki Miyazaki; Chihong Song; Filipe R N C Maia; Hemanth K N Reddy; Chantal Abergel; Jean-Michel Claverie; Janos Hajdu; Martin Svenda; Kazuyoshi Murata
Journal:  Sci Rep       Date:  2017-10-16       Impact factor: 4.379

8.  Local variations of HER2 dimerization in breast cancer cells discovered by correlative fluorescence and liquid electron microscopy.

Authors:  Diana B Peckys; Ulrike Korf; Niels de Jonge
Journal:  Sci Adv       Date:  2015-07-17       Impact factor: 14.136

9.  Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.

Authors:  Kem A Sochacki; Gleb Shtengel; Schuyler B van Engelenburg; Harald F Hess; Justin W Taraska
Journal:  Nat Methods       Date:  2014-01-26       Impact factor: 28.547

10.  New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography.

Authors:  Martin Schorb; Leander Gaechter; Ori Avinoam; Frank Sieckmann; Mairi Clarke; Cecilia Bebeacua; Yury S Bykov; Andreas F-P Sonnen; Reinhard Lihl; John A G Briggs
Journal:  J Struct Biol       Date:  2016-06-28       Impact factor: 2.867
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  9 in total

Review 1.  Elusive structure of mammalian DGKs.

Authors:  Qianqian Ma; Lakshmi Srinivasan; Sandra B Gabelli; Daniel M Raben
Journal:  Adv Biol Regul       Date:  2021-12-02

Review 2.  Find your coat: Using correlative light and electron microscopy to study intracellular protein coats.

Authors:  Kem A Sochacki; Justin W Taraska
Journal:  Curr Opin Cell Biol       Date:  2021-03-05       Impact factor: 8.386

3.  A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane.

Authors:  Shrawan Kumar Mageswaran; Wei Yuan Yang; Yogaditya Chakrabarty; Catherine M Oikonomou; Grant J Jensen
Journal:  Sci Adv       Date:  2021-03-26       Impact factor: 14.136

4.  CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.

Authors:  Jie E Yang; Matthew R Larson; Bryan S Sibert; Samantha Shrum; Elizabeth R Wright
Journal:  J Struct Biol       Date:  2021-02-18       Impact factor: 3.234

5.  Pattern Formation in Catalytic H2 Oxidation on Rh: Zooming in by Correlative Microscopy.

Authors:  Johannes Zeininger; Philipp Winkler; Maximilian Raab; Yuri Suchorski; Mauricio J Prieto; Liviu C Tănase; Lucas de Souza Caldas; Aarti Tiwari; Thomas Schmidt; Michael Stöger-Pollach; Andreas Steiger-Thirsfeld; Beatriz Roldan Cuenya; Günther Rupprechter
Journal:  ACS Catal       Date:  2022-09-19       Impact factor: 13.700

Review 6.  Structural Insights into TOR Signaling.

Authors:  Lucas Tafur; Jennifer Kefauver; Robbie Loewith
Journal:  Genes (Basel)       Date:  2020-08-04       Impact factor: 4.096

7.  Deriving and refining atomic models in crystallography and cryo-EM: the latest Phenix tools to facilitate structure analysis.

Authors:  Bruno P Klaholz
Journal:  Acta Crystallogr D Struct Biol       Date:  2019-10-01       Impact factor: 7.652

Review 8.  Recent Strategic Advances in CFTR Drug Discovery: An Overview.

Authors:  Marco Rusnati; Pasqualina D'Ursi; Nicoletta Pedemonte; Chiara Urbinati; Robert C Ford; Elena Cichero; Matteo Uggeri; Alessandro Orro; Paola Fossa
Journal:  Int J Mol Sci       Date:  2020-03-31       Impact factor: 5.923

Review 9.  Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy.

Authors:  Dokyung Jeong; Doory Kim
Journal:  Mol Cells       Date:  2022-01-31       Impact factor: 5.034
  9 in total

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