| Literature DB >> 17277781 |
Michael Marko1, Chyongere Hsieh, Richard Schalek, Joachim Frank, Carmen Mannella.
Abstract
Cryo-electron microscopy can provide high-resolution structural information about cells and organelles in the nearly native, frozen-hydrated state. Applicability, however, is limited by difficulties encountered in preparing suitably thin, vitreously frozen biological specimens. We demonstrate, by cryo-electron tomography of Escherichia coli cells, that a focused ion beam (FIB) can be used to thin whole frozen-hydrated cells in a convenient and essentially artifact-free way.Entities:
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Year: 2007 PMID： 17277781 DOI： 10.1038/nmeth1014
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547