Payam Behzadi1, Elham Behzadi. 1. MSc, Microbiology Department, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran - Iran.
Abstract
OBJECTIVES: Bacillus anthracis as the bacterial agent of anthrax, is spread in different geographical zones around the world.The purpose of this survey was to observe eventual apoptotic reactions in total genomic DNA of UVB irradiated colonies of Bacillus anthracis. MATERIALS AND METHODS: The colonies of Bacillus anthracis were exposed to UVB ray for 10 minutes; then, the DNA molecules of control and irradiated colonies were extracted. Finally, the DNA samples mixed in loading dye, were run in 1% agarose gel containing fluorescent dye of ethidium bromide to produce visible DNA bands. OUTCOMES: Neither smear nor DNA laddering band were detected upon the agarose gel. CONCLUSIONS: According to the present protocol, the UVB ray can not induce apoptosis feature in colonies of Bacillus anthracis. It seems that the recovery and protection systems in Bacillus anthracis can resist against eventual UVB disorders.
OBJECTIVES:Bacillus anthracis as the bacterial agent of anthrax, is spread in different geographical zones around the world.The purpose of this survey was to observe eventual apoptotic reactions in total genomic DNA of UVB irradiated colonies of Bacillus anthracis. MATERIALS AND METHODS: The colonies of Bacillus anthracis were exposed to UVB ray for 10 minutes; then, the DNA molecules of control and irradiated colonies were extracted. Finally, the DNA samples mixed in loading dye, were run in 1% agarose gel containing fluorescent dye of ethidium bromide to produce visible DNA bands. OUTCOMES: Neither smear nor DNA laddering band were detected upon the agarose gel. CONCLUSIONS: According to the present protocol, the UVB ray can not induce apoptosis feature in colonies of Bacillus anthracis. It seems that the recovery and protection systems in Bacillus anthracis can resist against eventual UVB disorders.
Entities:
Keywords:
Apoptosis; Bacillus anthracis; DNA; Gel electrophoresis
Authors: Alim M Aikembayev; Larissa Lukhnova; Gulnara Temiraliyeva; Tatyana Meka-Mechenko; Yerlan Pazylov; Sarkis Zakaryan; Georgiy Denissov; W Ryan Easterday; Matthew N Van Ert; Paul Keim; Stephen C Francesconi; Jason K Blackburn; Martin Hugh-Jones; Ted Hadfield Journal: Emerg Infect Dis Date: 2010-05 Impact factor: 6.883