Payam Behzadi1, Elham Behzadi. 1. MSc, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran.
Abstract
OBJECTIVES: The pathogenic disturbance of Saccharomyces cerevisiae is known as a rare but invasive nosocomial fungal infection. This survey is focused on the evaluation of apoptosis-triggering effects of UVB-irradiation in Saccharomyces cerevisiae. MATERIALS AND METHODS: The well-growth colonies of Saccharomyces cerevisiae on Sabouraud Dextrose Agar (SDA) were irradiated within an interval of 10 minutes by UVB-light (302 nm). Subsequently, the harvested DNA molecules of control and UV-exposed yeast colonies were run through the 1% agarose gel electrophoresis comprising the luminescent dye of ethidium bromide. OUTCOMES: No unusual patterns including DNA laddering bands or smears were detected. CONCLUSIONS: The applied procedure for UV exposure was not effective for inducing apoptosis in Saccharomyces cerevisiae. So, it needs another UV-radiation protocol for inducing apoptosis phenomenon in Saccharomyces cerevisiae.
OBJECTIVES: The pathogenic disturbance of Saccharomyces cerevisiae is known as a rare but invasive nosocomial fungal infection. This survey is focused on the evaluation of apoptosis-triggering effects of UVB-irradiation in Saccharomyces cerevisiae. MATERIALS AND METHODS: The well-growth colonies of Saccharomyces cerevisiae on Sabouraud Dextrose Agar (SDA) were irradiated within an interval of 10 minutes by UVB-light (302 nm). Subsequently, the harvested DNA molecules of control and UV-exposed yeast colonies were run through the 1% agarose gel electrophoresis comprising the luminescent dye of ethidium bromide. OUTCOMES: No unusual patterns including DNA laddering bands or smears were detected. CONCLUSIONS: The applied procedure for UV exposure was not effective for inducing apoptosis in Saccharomyces cerevisiae. So, it needs another UV-radiation protocol for inducing apoptosis phenomenon in Saccharomyces cerevisiae.
Authors: V Papaemmanouil; N Georgogiannis; M Plega; J Lalaki; D Lydakis; M Dimitriou; A Papadimitriou Journal: Anaerobe Date: 2011-04-29 Impact factor: 3.331