| Literature DB >> 21948410 |
Marco Gaspari1, Giovanni Cuda.
Abstract
Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel electrophoresis). Technical challenges, associated with low flow rates of the chromatographic separation, make this technology still difficult to run routinely. Here, we describe a nano LC-MS/MS setup that allows several weeks of continuous operation for the analysis of peptides derived by enzymatic digestion of either purified proteins or moderately complex protein mixtures.Mesh：
Year: 2011 PMID： 21948410 DOI： 10.1007/978-1-61779-319-6_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745