A Kensche1, S Pötschke2, C Hannig2, A Dürasch3, T Henle3, M Hannig4. 1. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany. Anna.Kensche@uniklinikum-dresden.de. 2. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstr. 74, 01307, Dresden, Germany. 3. Chair of Food Chemistry, TU Dresden, Dresden, Germany. 4. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, 66421, Homburg, Saarland, Germany.
Abstract
OBJECTIVES: The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the pellicle. MATERIALS/ METHODS: An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ pellicle. In situ pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm2). After 10 min of pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected pellicle samples were subjected to TEM analysis. RESULTS: All mouthrinses accumulated caseins in the in situ pellicle (2.0 ± 0.7-20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the pellicle. Milk homogenization also had an influence on the casein accumulation in the pellicle. TEM analysis confirmed the integration of micellar casein into the pellicle. CONCLUSION: The mouthrinses altered the protein composition and the ultrastructure of the in situ pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. CLINICAL RELEVANCE: The study provides interesting perspectives for innovative prevention strategies in dentistry.
OBJECTIVES: The adsorption of bovine milk caseins on the tooth surface might have a positive impact on the prevention of dental diseases. Therefore, the present study aimed to investigate the efficacy of mouthrinses with different types of bovine milk and milk protein isolates to accumulate caseins in the pellicle. MATERIALS/ METHODS: An indirect enzyme-linked immunosorbent assay (ELISA) was established to quantify the amount of caseins adsorbed into the in situ pellicle. In situ pellicle samples were collected from 2 volunteers on ceramic specimens (A = 8 cm2). After 10 min of pellicle formation, different types of bovine milk, 3% micellar casein in synthetic milk ultrafiltrate (SMUF) or 3% non-micellar caseinate in SMUF, were used as mouthrinses for 10 min. The pellicle material was harvested after 30 min in situ and examined for caseins by the indirect ELISA. Selected pellicle samples were subjected to TEM analysis. RESULTS: All mouthrinses accumulated caseins in the in situ pellicle (2.0 ± 0.7-20 ± 1.7 μg/ml) that, under native conditions, expressed no casein signal. Micellar protein association increased the adsorption of casein into the pellicle. Milk homogenization also had an influence on the casein accumulation in the pellicle. TEM analysis confirmed the integration of micellar casein into the pellicle. CONCLUSION: The mouthrinses altered the protein composition and the ultrastructure of the in situ pellicle to a different extent: bovine milk with 3.8% fat content and 3% micellar casein in SMUF being particularly effective. CLINICAL RELEVANCE: The study provides interesting perspectives for innovative prevention strategies in dentistry.
Entities:
Keywords:
Casein; ELISA; Lipids; Milk; Pellicle; TEM analysis
Authors: Jo E Frencken; Praveen Sharma; Laura Stenhouse; David Green; Dominic Laverty; Thomas Dietrich Journal: J Clin Periodontol Date: 2017-03 Impact factor: 8.728
Authors: Luiza P S Cassiano; Talita M S Ventura; Cintia M S Silva; Aline L Leite; Ana C Magalhães; Juliano P Pessan; Marília Afonso Rabelo Buzalaf Journal: Caries Res Date: 2018-01-27 Impact factor: 4.056
Authors: A Kensche; J Kirsch; S Mintert; F Enders; S Pötschke; S Basche; B König; C Hannig; M Hannig Journal: Sci Rep Date: 2017-11-29 Impact factor: 4.379
Authors: Ingegerd Johansson; Anders Esberg; Linda Eriksson; Simon Haworth; Pernilla Lif Holgerson Journal: PLoS One Date: 2018-03-21 Impact factor: 3.240