Literature DB >> 21936011

Comparative structural and functional characterization of putative protein effectors belonging to the PcF toxin family from Phytophthora spp.

Giuseppe Orsomando1, Lucia Brunetti, Kathleen Pucci, Barbara Ruggeri, Silverio Ruggieri.   

Abstract

The PcF Toxin Family (Pfam 09461) includes the characterized phytotoxic protein PcF from Phytophthora cactorum, as well as several predicted protein effectors from other Phytophthora species recently identified by comparative genomics. Here we provide first evidence that such 'putatives', recombinantly expressed in bacteria and purified to homogeneity, similarly to PcF, can trigger defense-related responses on tomato, that is leaf withering and phenylalanine ammonia lyase induction, although with various degrees of effectiveness. In addition, structural prediction by computer-aided homology modeling and subsequent structural/functional comparison after rational engineering of the disulfide-structured protein fold by site-directed mutagenesis, highlighted the surface-exposed conserved amino acid stretch SK(E/C)C as a possible structural determinant responsible for the differential phytotoxicity within this family of cognate protein effectors.
Copyright © 2011 The Protein Society.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21936011      PMCID： PMC3302648          DOI： 10.1002/pro.742

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  37 in total

1.  The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling.

Authors:  Konstantin Arnold; Lorenza Bordoli; Jürgen Kopp; Torsten Schwede
Journal:  Bioinformatics       Date:  2005-11-13       Impact factor: 6.937

Review 2.  Ten things to know about oomycete effectors.

Authors:  Sebastian Schornack; Edgar Huitema; Liliana M Cano; Tolga O Bozkurt; Ricardo Oliva; Mireille Van Damme; Simon Schwizer; Sylvain Raffaele; Angela Chaparro-Garcia; Rhys Farrer; Maria Eugenia Segretin; Jorunn Bos; Brian J Haas; Michael C Zody; Chad Nusbaum; Joe Win; Marco Thines; Sophien Kamoun
Journal:  Mol Plant Pathol       Date:  2009-11       Impact factor: 5.663

Review 3.  Fungal effector proteins: past, present and future.

Authors:  Pierre J G M De Wit; Rahim Mehrabi; Harrold A Van den Burg; Ioannis Stergiopoulos
Journal:  Mol Plant Pathol       Date:  2009-11       Impact factor: 5.663

4.  Initial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences.

Authors:  S Kamoun; P Hraber; B Sobral; D Nuss; F Govers
Journal:  Fungal Genet Biol       Date:  1999-11       Impact factor: 3.495

5.  PcF protein from Phytophthora cactorum and its recombinant homologue elicit phenylalanine ammonia lyase activation in tomato.

Authors:  G Orsomando; M Lorenzi; E Ferrari; C de Chiara; A Spisni; S Ruggieri
Journal:  Cell Mol Life Sci       Date:  2003-07       Impact factor: 9.261

Review 6.  Phytophthora ramorum: a pathogen with a remarkably wide host range causing sudden oak death on oaks and ramorum blight on woody ornamentals.

Authors:  Niklaus J Grünwald; Erica M Goss; Caroline M Press
Journal:  Mol Plant Pathol       Date:  2008-11       Impact factor: 5.663

7.  Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans.

Authors:  Brian J Haas; Sophien Kamoun; Michael C Zody; Rays H Y Jiang; Robert E Handsaker; Liliana M Cano; Manfred Grabherr; Chinnappa D Kodira; Sylvain Raffaele; Trudy Torto-Alalibo; Tolga O Bozkurt; Audrey M V Ah-Fong; Lucia Alvarado; Vicky L Anderson; Miles R Armstrong; Anna Avrova; Laura Baxter; Jim Beynon; Petra C Boevink; Stephanie R Bollmann; Jorunn I B Bos; Vincent Bulone; Guohong Cai; Cahid Cakir; James C Carrington; Megan Chawner; Lucio Conti; Stefano Costanzo; Richard Ewan; Noah Fahlgren; Michael A Fischbach; Johanna Fugelstad; Eleanor M Gilroy; Sante Gnerre; Pamela J Green; Laura J Grenville-Briggs; John Griffith; Niklaus J Grünwald; Karolyn Horn; Neil R Horner; Chia-Hui Hu; Edgar Huitema; Dong-Hoon Jeong; Alexandra M E Jones; Jonathan D G Jones; Richard W Jones; Elinor K Karlsson; Sridhara G Kunjeti; Kurt Lamour; Zhenyu Liu; Lijun Ma; Daniel Maclean; Marcus C Chibucos; Hayes McDonald; Jessica McWalters; Harold J G Meijer; William Morgan; Paul F Morris; Carol A Munro; Keith O'Neill; Manuel Ospina-Giraldo; Andrés Pinzón; Leighton Pritchard; Bernard Ramsahoye; Qinghu Ren; Silvia Restrepo; Sourav Roy; Ari Sadanandom; Alon Savidor; Sebastian Schornack; David C Schwartz; Ulrike D Schumann; Ben Schwessinger; Lauren Seyer; Ted Sharpe; Cristina Silvar; Jing Song; David J Studholme; Sean Sykes; Marco Thines; Peter J I van de Vondervoort; Vipaporn Phuntumart; Stephan Wawra; Rob Weide; Joe Win; Carolyn Young; Shiguo Zhou; William Fry; Blake C Meyers; Pieter van West; Jean Ristaino; Francine Govers; Paul R J Birch; Stephen C Whisson; Howard S Judelson; Chad Nusbaum
Journal:  Nature       Date:  2009-09-09       Impact factor: 49.962

8.  Patterns of diversifying selection in the phytotoxin-like scr74 gene family of Phytophthora infestans.

Authors:  Zhenyu Liu; Jorunn I B Bos; Miles Armstrong; Stephen C Whisson; Luis da Cunha; Trudy Torto-Alalibo; Joe Win; Anna O Avrova; Frank Wright; Paul R J Birch; Sophien Kamoun
Journal:  Mol Biol Evol       Date:  2004-11-17       Impact factor: 16.240

9.  Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis.

Authors:  Brett M Tyler; Sucheta Tripathy; Xuemin Zhang; Paramvir Dehal; Rays H Y Jiang; Andrea Aerts; Felipe D Arredondo; Laura Baxter; Douda Bensasson; Jim L Beynon; Jarrod Chapman; Cynthia M B Damasceno; Anne E Dorrance; Daolong Dou; Allan W Dickerman; Inna L Dubchak; Matteo Garbelotto; Mark Gijzen; Stuart G Gordon; Francine Govers; Niklaus J Grunwald; Wayne Huang; Kelly L Ivors; Richard W Jones; Sophien Kamoun; Konstantinos Krampis; Kurt H Lamour; Mi-Kyung Lee; W Hayes McDonald; Mónica Medina; Harold J G Meijer; Eric K Nordberg; Donald J Maclean; Manuel D Ospina-Giraldo; Paul F Morris; Vipaporn Phuntumart; Nicholas H Putnam; Sam Rash; Jocelyn K C Rose; Yasuko Sakihama; Asaf A Salamov; Alon Savidor; Chantel F Scheuring; Brian M Smith; Bruno W S Sobral; Astrid Terry; Trudy A Torto-Alalibo; Joe Win; Zhanyou Xu; Hongbin Zhang; Igor V Grigoriev; Daniel S Rokhsar; Jeffrey L Boore
Journal:  Science       Date:  2006-09-01       Impact factor: 47.728

10.  Analyses of genome architecture and gene expression reveal novel candidate virulence factors in the secretome of Phytophthora infestans.

Authors:  Sylvain Raffaele; Joe Win; Liliana M Cano; Sophien Kamoun
Journal:  BMC Genomics       Date:  2010-11-16       Impact factor: 3.969
View more
  4 in total

1.  Transcription profiling and identification of infection-related genes in Phytophthora cactorum.

Authors:  Xiao-Ren Chen; Shen-Xin Huang; Ye Zhang; Gui-Lin Sheng; Bo-Yue Zhang; Qi-Yuan Li; Feng Zhu; Jing-You Xu
Journal:  Mol Genet Genomics       Date:  2017-12-07       Impact factor: 3.291

2.  Transcriptomic analysis of the phytopathogenic oomycete Phytophthora cactorum provides insights into infection-related effectors.

Authors:  Xiao-Ren Chen; Bo-Yue Zhang; Yu-Ping Xing; Qi-Yuan Li; Yan-Peng Li; Yun-Hui Tong; Jing-You Xu
Journal:  BMC Genomics       Date:  2014-11-18       Impact factor: 3.969

3.  Molecular modeling of NK-CT1, from Indian monocellate cobra (Naja kaouthia) and its docking interaction with human DNA topoisomerase II alpha.

Authors:  Pathikrit Bandopadhyay; Soma Halder; Mrinmoy Sarkar; Sujay Kumar Bhunia; Sananda Dey; Antony Gomes; Biplab Giri
Journal:  Bioinformation       Date:  2016-06-15

4.  Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum.

Authors:  Andrew D Armitage; Erik Lysøe; Charlotte F Nellist; Laura A Lewis; Liliana M Cano; Richard J Harrison; May B Brurberg
Journal:  PLoS One       Date:  2018-10-02       Impact factor: 3.240
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.