Literature DB >> 21933706

A rapid fluorescence assay for hSMUG1 activity based on modified molecular beacon.

Xue Yang1, Chunyi Tong, Ying Long, Bin Liu.   

Abstract

A new method for assay of hSMUG1 in real-time using molecular beacon is reported. hSMUG1 could be detected linearly in the range from 0.67 nM to 10.05 nM and the detection limit is 0.168 nM. In addition, this method was applied to detect the activity of hSMUG1 in tumor cells and study kinetics. The probe with low background signal has been shown to be suitable for the real-time monitoring of hSMUG1 activity, making this a promising method of high-throughput clinical sample analysis. Copyright Â
© 2011 Elsevier Ltd. All rights reserved.

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Year:  2011        PMID: 21933706     DOI: 10.1016/j.mcp.2011.09.001

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN: 0890-8508            Impact factor:   2.365


  4 in total

1.  Quantitative, real-time analysis of base excision repair activity in cell lysates utilizing lesion-specific molecular beacons.

Authors:  David Svilar; Conchita Vens; Robert W Sobol
Journal:  J Vis Exp       Date:  2012-08-06       Impact factor: 1.355

2.  Expanding targets of DNAzyme-based sensors through deactivation and activation of DNAzymes by single uracil removal: sensitive fluorescent assay of uracil-DNA glycosylase.

Authors:  Yu Xiang; Yi Lu
Journal:  Anal Chem       Date:  2012-10-29       Impact factor: 6.986

Review 3.  Fluorescent Probes of DNA Repair.

Authors:  David L Wilson; Eric T Kool
Journal:  ACS Chem Biol       Date:  2017-11-30       Impact factor: 5.100

4.  Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening.

Authors:  Avani Mehta; Prateek Raj; Sandeep Sundriyal; Balasubramanian Gopal; Umesh Varshney
Journal:  Biochem Biophys Rep       Date:  2021-02-15
  4 in total

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