BACKGROUND: Cytomegalovirus (CMV), the most common cause of congenital infection, exhibits extensive genetic variability. We sought to determine whether multiple CMV strains can be transmitted to the fetus and to describe the distribution of genotypes in the saliva, urine, and blood. METHODS: Study subjects consisted of a convenience sampling of 28 infants found to be CMV-positive on newborn screening as part of an ongoing study. Genotyping was performed on saliva specimens obtained during newborn screening and urine, saliva, and blood obtained at a later time point within the first 3 weeks of life. RESULTS: Six (21.4%) of the 28 saliva samples obtained within the first 2 days of life contained >1 CMV genotype. Multiple CMV genotypes were found in 39% (5/13) of urine, saliva, and blood samples obtained within the first 3 weeks of life from 13 of the 28 newborns. There was no predominance of a CMV genotype at a specific site; however, 4 infants demonstrated distinct CMV strains in different compartments. CONCLUSIONS: Infection with multiple CMV strains occurs in infants with congenital CMV infection. The impact of intrauterine infection with multiple virus strains on the pathogenesis and long-term outcome remains to be elucidated.
BACKGROUND: Cytomegalovirus (CMV), the most common cause of congenital infection, exhibits extensive genetic variability. We sought to determine whether multiple CMV strains can be transmitted to the fetus and to describe the distribution of genotypes in the saliva, urine, and blood. METHODS: Study subjects consisted of a convenience sampling of 28 infants found to be CMV-positive on newborn screening as part of an ongoing study. Genotyping was performed on saliva specimens obtained during newborn screening and urine, saliva, and blood obtained at a later time point within the first 3 weeks of life. RESULTS: Six (21.4%) of the 28 saliva samples obtained within the first 2 days of life contained >1 CMV genotype. Multiple CMV genotypes were found in 39% (5/13) of urine, saliva, and blood samples obtained within the first 3 weeks of life from 13 of the 28 newborns. There was no predominance of a CMV genotype at a specific site; however, 4 infants demonstrated distinct CMV strains in different compartments. CONCLUSIONS:Infection with multiple CMV strains occurs in infants with congenital CMV infection. The impact of intrauterine infection with multiple virus strains on the pathogenesis and long-term outcome remains to be elucidated.
Authors: Elisabeth Puchhammer-Stöckl; Irene Görzer; Alexander Zoufaly; Peter Jaksch; Claudia C Bauer; Walter Klepetko; Therese Popow-Kraupp Journal: Transplantation Date: 2006-01-27 Impact factor: 4.939
Authors: Nicholas Renzette; Cornelia Pokalyuk; Laura Gibson; Bornali Bhattacharjee; Mark R Schleiss; Klaus Hamprecht; Aparecida Y Yamamoto; Marisa M Mussi-Pinhata; William J Britt; Jeffrey D Jensen; Timothy F Kowalik Journal: Proc Natl Acad Sci U S A Date: 2015-07-06 Impact factor: 11.205
Authors: Yaping Liu; Daniel C Freed; Leike Li; Aimin Tang; Fengsheng Li; Edward M Murray; Stuart P Adler; Michael A McVoy; Richard E Rupp; Diane Barrett; Xiaohua Ye; Ningyan Zhang; Karen Beck; Timothy Culp; Rituparna Das; Liping Song; Kalpit Vora; Hua Zhu; Dai Wang; Amy S Espeseth; Zhiqiang An; Luwy Musey; Tong-Ming Fu Journal: J Virol Date: 2019-11-13 Impact factor: 5.103