PURPOSE: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. PROCEDURES: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. RESULTS: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. CONCLUSIONS: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
PURPOSE: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. PROCEDURES: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. RESULTS: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. CONCLUSIONS: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.
Authors: N Ikeda; H Honda; T Katsumi; T Okunaka; K Furukawa; T Tsuchida; K Tanaka; T Onoda; T Hirano; M Saito; N Kawate; C Konaka; H Kato; Y Ebihara Journal: Diagn Ther Endosc Date: 1999
Authors: Hasmik Agadjanian; David Chu; Jae Youn Hwang; Sebastian Wachsmann-Hogiu; Altan Rentsendorj; Lei Song; Vinod Valluripalli; Jay Lubow; Jun Ma; Behrooz Sharifi; Daniel L Farkas; Lali K Medina-Kauwe Journal: Nanomedicine (Lond) Date: 2012-03 Impact factor: 5.307
Authors: Michael A Pinkert; Lonie R Salkowski; Patricia J Keely; Timothy J Hall; Walter F Block; Kevin W Eliceiri Journal: J Med Imaging (Bellingham) Date: 2018-01-22
Authors: Jae Youn Hwang; David J Lubow; David Chu; Jessica Sims; Felix Alonso-Valenteen; Harry B Gray; Zeev Gross; Daniel L Farkas; Lali K Medina-Kauwe Journal: J Control Release Date: 2012-10-04 Impact factor: 9.776
Authors: Gerardo J Ramírez-Nava; Clara L Santos-Cuevas; Isaac Chairez; Liliana Aranda-Lara Journal: Med Biol Eng Comput Date: 2017-12-01 Impact factor: 2.602