BACKGROUND: We investigate whether optical imaging can reliably detect abnormalities in tissue, in a range of specimens (live cells in vitro; fixed, fresh ex-vivo and in vivo tissue), without the use of added contrast agents, and review our promising spectral methods for achieving quantitative, real-time, high resolution intrasurgical optical diagnostics. METHODS: We use reflectance, fluorescence, two-photon, and Mie scattering imaging, performed with instrumentation we developed or modified, to detect intrinsic tissue signatures. Emphasis is on spectral/hyperspectral imaging approaches allowing the equivalent of in vivo pathology. RESULTS: With experimental focus on unstained specimens, we demonstrate the ability to segment tissue images for cancer detection. Spectral reflectance imaging, coupled with advanced analysis, typically yields 90% specificity and sensitivity. Autofluorescence is also shown to be diagnostically useful, with lymph nodes results highlighted here. Elastic scattering hyperspectral imaging endoscopy, using a new instrument we designed and built, shows promise in bronchoscopic detection of dysplasia and early cancer in patients. CONCLUSIONS: The results demonstrate that advanced optical imaging can detect and localize cellular signatures of cancer in real-time, in vivo, without the use of contrast agents, in animals and humans. This is an important step towards tight spatio-temporal coupling between such detection and clinical intervention. (c) 2006 International Society for Analytical Cytology.
BACKGROUND: We investigate whether optical imaging can reliably detect abnormalities in tissue, in a range of specimens (live cells in vitro; fixed, fresh ex-vivo and in vivo tissue), without the use of added contrast agents, and review our promising spectral methods for achieving quantitative, real-time, high resolution intrasurgical optical diagnostics. METHODS: We use reflectance, fluorescence, two-photon, and Mie scattering imaging, performed with instrumentation we developed or modified, to detect intrinsic tissue signatures. Emphasis is on spectral/hyperspectral imaging approaches allowing the equivalent of in vivo pathology. RESULTS: With experimental focus on unstained specimens, we demonstrate the ability to segment tissue images for cancer detection. Spectral reflectance imaging, coupled with advanced analysis, typically yields 90% specificity and sensitivity. Autofluorescence is also shown to be diagnostically useful, with lymph nodes results highlighted here. Elastic scattering hyperspectral imaging endoscopy, using a new instrument we designed and built, shows promise in bronchoscopic detection of dysplasia and early cancer in patients. CONCLUSIONS: The results demonstrate that advanced optical imaging can detect and localize cellular signatures of cancer in real-time, in vivo, without the use of contrast agents, in animals and humans. This is an important step towards tight spatio-temporal coupling between such detection and clinical intervention. (c) 2006 International Society for Analytical Cytology.
Authors: Peter F Favreau; Clarissa Hernandez; Tiffany Heaster; Diego F Alvarez; Thomas C Rich; Prashant Prabhat; Silas J Leavesley Journal: J Biomed Opt Date: 2014-04 Impact factor: 3.170
Authors: Jae Youn Hwang; Jay Lubow; David Chu; Zeev Gross; Harry B Gray; Daniel L Farkas; Lali K Medina-Kauwe Journal: Proc SPIE Int Soc Opt Eng Date: 2011-01-22
Authors: Jae Youn Hwang; Sebastian Wachsmann-Hogiu; V Krishnan Ramanujan; Julia Ljubimova; Zeev Gross; Harry B Gray; Lali K Medina-Kauwe; Daniel L Farkas Journal: Mol Imaging Biol Date: 2012-08 Impact factor: 3.488
Authors: Jae Youn Hwang; David J Lubow; Jessica D Sims; Harry B Gray; Atif Mahammed; Zeev Gross; Lali K Medina-Kauwe; Daniel L Farkas Journal: J Biomed Opt Date: 2012-01 Impact factor: 3.170