Literature DB >> 2182373

Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation.

J Augustin1, F Götz.   

Abstract

In this paper, the influence of various parameters on plasmid transformation by electroporation of Staphylococcus epidermidis Tü3298 was investigated. Cell growth conditions, various concentrations and forms of plasmid DNA, field strength, pulse duration and media for electroporation and regeneration were tested. In order to obtain optimal transformation efficiency, the cells were incubated for 30 min with DNA before pulsing. With the optimized procedure, other staphylococcal species such as S. aureus, S. staphylolyticus and S. carnosus were transformed with an efficiency up to 3 X 10(5) transformants per micrograms pC194 plasmid DNA.

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Year:  1990        PMID： 2182373     DOI： 10.1016/0378-1097(90)90283-v

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  102 in total

1.  Identification of the sigB operon in Staphylococcus epidermidis: construction and characterization of a sigB deletion mutant.

Authors:  S Kies; M Otto; C Vuong; F Götz
Journal:  Infect Immun       Date:  2001-12       Impact factor: 3.441

2.  New erm Gene in Staphylococcus aureus clinical isolates.

Authors:  Mayumi Matsuoka; Matsuhisa Inoue; Yoshinori Nakajima; Yoshihiro Endo
Journal:  Antimicrob Agents Chemother       Date:  2002-01       Impact factor: 5.191

3.  The nitrate reductase and nitrite reductase operons and the narT gene of Staphylococcus carnosus are positively controlled by the novel two-component system NreBC.

Authors:  I Fedtke; A Kamps; B Krismer; F Götz
Journal:  J Bacteriol       Date:  2002-12       Impact factor: 3.490

4.  Localization and functional analysis of PepI, the immunity peptide of Pep5-producing Staphylococcus epidermidis strain 5.

Authors:  Anja Hoffmann; Tanja Schneider; Ulrike Pag; Hans-Georg Sahl
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

5.  Molecular characterization of the nitrite-reducing system of Staphylococcus carnosus.

Authors:  H Neubauer; I Pantel; F Götz
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

6.  Regulation of Staphylococcus aureus type 5 and type 8 capsular polysaccharides by CO(2).

Authors:  S Herbert; S W Newell; C Lee; K P Wieland; B Dassy; J M Fournier; C Wolz; G Döring
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

7.  CspA regulates pigment production in Staphylococcus aureus through a SigB-dependent mechanism.

Authors:  Samuel Katzif; Eun-Hee Lee; Anthony B Law; Yih-Ling Tzeng; William M Shafer
Journal:  J Bacteriol       Date:  2005-12       Impact factor: 3.490

8.  VraSR two-component regulatory system and its role in induction of pbp2 and vraSR expression by cell wall antimicrobials in Staphylococcus aureus.

Authors:  Shaohui Yin; Robert S Daum; Susan Boyle-Vavra
Journal:  Antimicrob Agents Chemother       Date:  2006-01       Impact factor: 5.191

9.  Transcriptional Regulation of icaADBC by both IcaR and TcaR in Staphylococcus epidermidis.

Authors:  Tra-My Hoang; C Zhou; J K Lindgren; M R Galac; B Corey; J E Endres; M E Olson; P D Fey
Journal:  J Bacteriol       Date:  2019-02-25       Impact factor: 3.490

10.  Virulence of Staphylococcus aureus mutants altered in type 5 capsule production.

Authors:  A Albus; R D Arbeit; J C Lee
Journal:  Infect Immun       Date:  1991-03       Impact factor: 3.441
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