Literature DB >> 2178785

Transformation of Aspergillus giganteus to hygromycin B resistance.

S Wnendt1, M Jacobs, U Stahl.   

Abstract

A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per microgram DNA per 3 x 10(3) viable protoplasts. Vector DNA could be recovered by transformation of Escherichia coli with undigested genomic DNA from Aspergillus giganteus transformants.

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Year:  1990        PMID： 2178785     DOI： 10.1007/BF00313244

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  23 in total

1.  Mitotic gene conversion, reciprocal recombination and gene replacement at the benA, beta-tubulin, locus of Aspergillus nidulans.

Authors:  P W Dunne; B R Oakley
Journal:  Mol Gen Genet       Date:  1988-08

2.  Isolation of a phytoalexin-detoxification gene from the plant pathogenic fungus Nectria haematococca by detecting its expression in Aspergillus nidulans.

Authors:  K M Weltring; B G Turgeon; O C Yoder; H D VanEtten
Journal:  Gene       Date:  1988-09-07       Impact factor: 3.688

3.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

4.  Evaluation of mitogillin (NSC-69529) in the treatment of naturally occurring canine neoplasms.

Authors:  V Roga; L P Hedeman; B H Olson
Journal:  Cancer Chemother Rep       Date:  1971-04

5.  Experimental screening procedures and clinical predictability value.

Authors:  A Goldin; A A Serpick; N Mantel
Journal:  Cancer Chemother Rep       Date:  1966-05

6.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

7.  ALPHA SARCIN, A NEW ANTITUMOR AGENT. I. ISOLATION, PURIFICATION, CHEMICAL COMPOSITION, AND THE IDENTITY OF A NEW AMINO ACID.

Authors:  B H OLSON; G L GOERNER
Journal:  Appl Microbiol       Date:  1965-05

8.  ALPHA SARCIN, A NEW ANTITUMOR AGENT. II. FERMENTATION AND ANTITUMOR SPECTRUM.

Authors:  J C JENNINGS; B H OLSON; V ROGA; A J JUNEK; D M SCHUURMANS
Journal:  Appl Microbiol       Date:  1965-05

9.  Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene.

Authors:  K Wernars; T Goosen; L M Wennekes; J Visser; C J Bos; H W van den Broek; R F van Gorcom; C A van den Hondel; P H Pouwels
Journal:  Curr Genet       Date:  1985       Impact factor: 3.886

10.  Cloning an Aspergillus nidulans developmental gene by transformation.

Authors:  I L Johnstone; S G Hughes; A J Clutterbuck
Journal:  EMBO J       Date:  1985-05       Impact factor: 11.598
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  10 in total

1.  Integrative transformation by homologous recombination in the zygomycete Mucor circinelloides.

Authors:  J Arnau; L P Jepsen; P Strøman
Journal:  Mol Gen Genet       Date:  1991-02

2.  Transformation of a methionine auxotrophic mutant of Mucor circinelloides by direct cloning of the corresponding wild type gene.

Authors:  N Anaya; M I Roncero
Journal:  Mol Gen Genet       Date:  1991-12

3.  Cloning and nucleotide sequence of a cDNA encoding the antifungal-protein of Aspergillus giganteus and preliminary characterization of the native gene.

Authors:  S Wnendt; N Ulbrich; U Stahl
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

4.  Surrogate transformation of perennial ryegrass, Lolium perenne, using genetically modified Acremonium endophyte.

Authors:  F R Murray; G C Latch; D B Scott
Journal:  Mol Gen Genet       Date:  1992-05

5.  Altered expression of the steroid bioconverting pathway in pAN 7-1 transformants of Cochliobolus lunatus.

Authors:  D Rozman; R Komel
Journal:  Curr Genet       Date:  1991-11       Impact factor: 3.886

6.  Comparison of different transformation methods for Aspergillus giganteus.

Authors:  Vera Meyer; Dirk Mueller; Till Strowig; Ulf Stahl
Journal:  Curr Genet       Date:  2003-05-17       Impact factor: 3.886

7.  Heterologous transformation of Zalerion arboricola.

Authors:  R Kelly; E Register; M Sosa
Journal:  Curr Genet       Date:  1994-09       Impact factor: 3.886

8.  Transformation frequencies are enhanced and vector DNA is targeted during retransformation of Leptosphaeria maculans, a fungal plant pathogen.

Authors:  M L Farman; R P Oliver
Journal:  Mol Gen Genet       Date:  1992-01

9.  Transformations of Penicillium islandicum and Penicillium frequentans that produce anthraquinone-related compounds.

Authors:  K X Huang; N Iwakami; I Fujii; Y Ebizuka; U Sankawa
Journal:  Curr Genet       Date:  1995-11       Impact factor: 3.886

10.  Identification of Novel Mutations Contributing to Azole Tolerance of Aspergillus fumigatus through In Vitro Exposure to Tebuconazole.

Authors:  Takahito Toyotome; Kenji Onishi; Mio Sato; Yoko Kusuya; Daisuke Hagiwara; Akira Watanabe; Hiroki Takahashi
Journal:  Antimicrob Agents Chemother       Date:  2021-08-17       Impact factor: 5.191
  10 in total

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