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Literature DB >> 3916728

Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene.

K Wernars¹, T Goosen, L M Wennekes, J Visser, C J Bos, H W van den Broek, R F van Gorcom, C A van den Hondel, P H Pouwels.

Abstract

Conidial protoplasts of an A. nidulans amdS deletion strain (MH1277) have been transformed to the AmdS+ phenotype with a plasmid carrying the wild type gene (p3SR2). Optimalisation of transformation and plating conditions now has resulted in frequencies of 300-400 transformants per microgram of DNA. Analysis of DNA from AmdS+ transformants of MH1277 showed that transformation had occurred by integration of vector DNA sequences into the genome. In virtually all these transformants multiple copies of the vector were present in a tandemly repeated fashion, not preferentially at the resident, partially deleted amdS gene. It is suggested that the observed integration phenomena are dependent on the genetic background of the A. nidulans strain, used for transformation. A model to explain the tandem type of integration is proposed.

Entities: Species

Mesh：

Year: 1985 PMID： 3916728 DOI： 10.1007/bf00421606

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

24 in total

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Journal: Mol Cell Biol Date: 1983-08 Impact factor: 4.272

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Authors: M J Hynes
Journal: Genetics Date: 1982-10 Impact factor: 4.562

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Authors: C J Bos; S M Slakhorst
Journal: Can J Microbiol Date: 1981-04 Impact factor: 2.419

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Journal: Proc Natl Acad Sci U S A Date: 1984-03 Impact factor: 11.205

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Journal: Nature Date: 1980-10-30 Impact factor: 49.962

10. Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors: M E Case; M Schweizer; S R Kushner; N H Giles
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

34 in total

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Journal: Mol Cell Biol Date: 1991-02 Impact factor: 4.272

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Journal: Genetics Date: 1989-06 Impact factor: 4.562

5. Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

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Journal: Curr Genet Date: 1986 Impact factor: 3.886

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Authors: P van den Broek; T Goosen; B Wennekes; H van den Broek
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8. Two novel, putatively cell wall-associated and glycosylphosphatidylinositol-anchored alpha-glucanotransferase enzymes of Aspergillus niger.

Authors: R M van der Kaaij; X-L Yuan; A Franken; A F J Ram; P J Punt; M J E C van der Maarel; L Dijkhuizen
Journal: Eukaryot Cell Date: 2007-05-11

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Journal: Curr Genet Date: 1993 Impact factor: 3.886

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Authors: Y Itoh; R Johnson; B Scott
Journal: Curr Genet Date: 1994-06 Impact factor: 3.886