Literature DB >> 12791907

Stability of repetitive-sequence PCR patterns with respect to culture age and subculture frequency.

Hyunseok Peter Kang1, W Michael Dunne.   

Abstract

To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35 degrees C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. rep PCR analysis demonstrated that all rep PCR fingerprints from a single species were identical.

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Year:  2003        PMID： 12791907      PMCID： PMC156572          DOI： 10.1128/JCM.41.6.2694-2696.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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