Literature DB >> 14532224

Optimization of real-time PCR assay for rapid and sensitive detection of eubacterial 16S ribosomal DNA in platelet concentrates.

Tamimount Mohammadi1, Henk W Reesink, Christina M J E Vandenbroucke-Grauls, Paul H M Savelkoul.   

Abstract

A real-time PCR assay was developed for rapid detection of eubacterial 16S ribosomal DNA in platelet concentrates. The sensitivity of this assay can be hampered by contaminating DNA in the PCR reagents. Digestion of the PCR reagents with Sau3AI prior to PCR amplification was effective in eliminating this contaminating DNA without affecting the sensitivity of the assay.

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Year:  2003        PMID： 14532224      PMCID： PMC254335          DOI： 10.1128/JCM.41.10.4796-4798.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

2.  Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set.

Authors:  Mangala A Nadkarni; F Elizabeth Martin; Nicholas A Jacques; Neil Hunter
Journal:  Microbiology       Date:  2002-01       Impact factor: 2.777

Review 3.  Bacterial contamination of platelet concentrates: incidence, significance, and prevention.

Authors:  M A Blajchman; M Goldman
Journal:  Semin Hematol       Date:  2001-10       Impact factor: 3.851

Review 4.  Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.

Authors:  B Cherie Millar; Jiru Xu; John E Moore
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

5.  Contamination management of broad-range ribosomal DNA PCR: where is the evidence?

Authors:  Stefaan J Vandecasteele; Johan Frans; Marc Van Ranst
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

6.  Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens.

Authors:  Samuel Yang; Shin Lin; Gabor D Kelen; Thomas C Quinn; James D Dick; Charlotte A Gaydos; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

7.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

8.  Avoiding false positives with PCR.

Authors:  S Kwok; R Higuchi
Journal:  Nature       Date:  1989-05-18       Impact factor: 49.962

9.  Detection of bacteraemia in critically ill patients using 16S rDNA polymerase chain reaction and DNA sequencing.

Authors:  J Sleigh; R Cursons; M La Pine
Journal:  Intensive Care Med       Date:  2001-08       Impact factor: 17.440

10.  Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.

Authors:  N M Carroll; P Adamson; N Okhravi
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948
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  25 in total

1.  Reduced PCR sensitivity due to impaired DNA recovery with the MagNA Pure LC total nucleic acid isolation kit.

Authors:  Tim Schuurman; Alex van Breda; Richard de Boer; Mirjam Kooistra-Smid; Marcel Beld; Paul Savelkoul; René Boom
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

2.  Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Authors:  Franziska Zucol; Roland A Ammann; Christoph Berger; Christoph Aebi; Martin Altwegg; Felix K Niggli; David Nadal
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

3.  Implementation of Bacterial Detection Methods into Blood Donor Screening - Overview of Different Technologies.

Authors:  Michael Schmidt; Walid Sireis; Erhard Seifried
Journal:  Transfus Med Hemother       Date:  2011-07-07       Impact factor: 3.747

4.  A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.

Authors:  Hideki Niimi; Masashi Mori; Homare Tabata; Hiroshi Minami; Tomohiro Ueno; Shirou Hayashi; Isao Kitajima
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

5.  Establishment of a method for measuring leukocyte phagocytosis based on 16S rDNA.

Authors:  Haiyan Zhou; Wenping Sun; Hui Liu
Journal:  Mol Biol Rep       Date:  2018-12-01       Impact factor: 2.316

6.  Enhancement of PCR Detection Limit by Single-Tube Restriction Endonuclease-PCR (RE-PCR).

Authors:  Sibnarayan Datta; Raghvendra Budhauliya; Soumya Chatterjee; Vijay Veer; Runu Chakravarty
Journal:  Mol Diagn Ther       Date:  2016-06       Impact factor: 4.074

7.  Multimodal Magneto-Fluorescent Nanosensor for Rapid and Specific Detection of Blood-Borne Pathogens.

Authors:  Tuhina Banerjee; Tanuja Tummala; Rebekah Elliott; Vedant Jain; Wesley Brantley; Laci Hadorn; Santimukul Santra
Journal:  ACS Appl Nano Mater       Date:  2019-08-19

8.  The effect of rifaximin on gut flora and Staphylococcus resistance.

Authors:  Mi-Sung Kim; Walter Morales; Andres Ardila Hani; Sharon Kim; Gene Kim; Stacy Weitsman; Christopher Chang; Mark Pimentel
Journal:  Dig Dis Sci       Date:  2013-04-16       Impact factor: 3.199

9.  An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.

Authors:  Sophie Champlot; Camille Berthelot; Mélanie Pruvost; E Andrew Bennett; Thierry Grange; Eva-Maria Geigl
Journal:  PLoS One       Date:  2010-09-28       Impact factor: 3.240

10.  Optimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteria.

Authors:  Rudolph Spangler; Noel L Goddard; David S Thaler
Journal:  PLoS One       Date:  2009-09-15       Impact factor: 3.240
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