BACKGROUND: Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable in vitro tests for predicting the skin sensitizing potentials of a wide variety of industrial chemicals. Previously, we developed the human cell line activation test (h-CLAT) as a cell-based assay to predict the skin sensitizing potential of chemicals, and showed the correspondence between the h-CLAT and the murine local lymph node assay results. OBJECTIVES: This study was conducted to investigate the predictive performance of the h-CLAT for human skin sensitizing potential. MATERIALS/ METHODS: We selected a total of 66 test chemicals with known human sensitizing potential, and tested all chemicals with the h-CLAT. We then evaluated the performance of the h-CLAT in predicting human sensitizing potential. RESULTS AND CONCLUSION: Forty-five of 51 tested sensitizers were positive in the h-CLAT, indicating relatively high sensitivity. Also, 10 of 15 non-sensitizers were correctly detected as negative. The overall agreement between human data and h-CLAT outcome was 83%. Furthermore, the h-CLAT could accurately predict the human sensitizing potential of 23 tested chemicals that were amines, heterocyclic compounds, or sulfur compounds. Our data indicate the utility of the h-CLAT for predicting the human skin sensitizing potential of a variety of chemicals.
BACKGROUND: Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable in vitro tests for predicting the skin sensitizing potentials of a wide variety of industrial chemicals. Previously, we developed the human cell line activation test (h-CLAT) as a cell-based assay to predict the skin sensitizing potential of chemicals, and showed the correspondence between the h-CLAT and the murine local lymph node assay results. OBJECTIVES: This study was conducted to investigate the predictive performance of the h-CLAT for human skin sensitizing potential. MATERIALS/ METHODS: We selected a total of 66 test chemicals with known human sensitizing potential, and tested all chemicals with the h-CLAT. We then evaluated the performance of the h-CLAT in predicting human sensitizing potential. RESULTS AND CONCLUSION: Forty-five of 51 tested sensitizers were positive in the h-CLAT, indicating relatively high sensitivity. Also, 10 of 15 non-sensitizers were correctly detected as negative. The overall agreement between human data and h-CLAT outcome was 83%. Furthermore, the h-CLAT could accurately predict the human sensitizing potential of 23 tested chemicals that were amines, heterocyclic compounds, or sulfur compounds. Our data indicate the utility of the h-CLAT for predicting the human skin sensitizing potential of a variety of chemicals.
Authors: Candice Johnson; Ernst Ahlberg; Lennart T Anger; Lisa Beilke; Romualdo Benigni; Joel Bercu; Sol Bobst; David Bower; Alessandro Brigo; Sarah Campbell; Mark T D Cronin; Ian Crooks; Kevin P Cross; Tatyana Doktorova; Thomas Exner; David Faulkner; Ian M Fearon; Markus Fehr; Shayne C Gad; Véronique Gervais; Amanda Giddings; Susanne Glowienke; Barry Hardy; Catrin Hasselgren; Jedd Hillegass; Robert Jolly; Eckart Krupp; Liat Lomnitski; Jason Magby; Jordi Mestres; Lawrence Milchak; Scott Miller; Wolfgang Muster; Louise Neilson; Rahul Parakhia; Alexis Parenty; Patricia Parris; Alexandre Paulino; Ana Theresa Paulino; David W Roberts; Harald Schlecker; Reinhard Stidl; Diana Suarez-Rodrigez; David T Szabo; Raymond R Tice; Daniel Urbisch; Anna Vuorinen; Brian Wall; Thibaud Weiler; Angela T White; Jessica Whritenour; Joerg Wichard; David Woolley; Craig Zwickl; Glenn J Myatt Journal: Regul Toxicol Pharmacol Date: 2020-07-01 Impact factor: 3.271
Authors: Mihwa Na; Devin O'Brien; Maura Lavelle; Isabelle Lee; G Frank Gerberick; Anne Marie Api Journal: Dermatitis Date: 2022 Mar-Apr 01 Impact factor: 4.867
Authors: Andy Forreryd; Henrik Johansson; Ann-Sofie Albrekt; Carl A K Borrebaeck; Malin Lindstedt Journal: PLoS One Date: 2015-03-11 Impact factor: 3.240