Literature DB >> 21730057

A conserved asparagine residue in transmembrane segment 1 (TM1) of serotonin transporter dictates chloride-coupled neurotransmitter transport.

L Keith Henry1, Hideki Iwamoto2, Julie R Field2, Kristian Kaufmann3, Eric S Dawson4, Miriam T Jacobs5, Chelsea Adams6, Bruce Felts6, Igor Zdravkovic7, Vanessa Armstrong6, Steven Combs3, Ernesto Solis2, Gary Rudnick5, Sergei Y Noskov7, Louis J DeFelice8, Jens Meiler9, Randy D Blakely10.   

Abstract

Na(+)- and Cl(-)-dependent uptake of neurotransmitters via transporters of the SLC6 family, including the human serotonin transporter (SLC6A4), is critical for efficient synaptic transmission. Although residues in the human serotonin transporter involved in direct Cl(-) coordination of human serotonin transport have been identified, the role of Cl(-) in the transport mechanism remains unclear. Through a combination of mutagenesis, chemical modification, substrate and charge flux measurements, and molecular modeling studies, we reveal an unexpected role for the highly conserved transmembrane segment 1 residue Asn-101 in coupling Cl(-) binding to concentrative neurotransmitter uptake.

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Year:  2011        PMID： 21730057      PMCID： PMC3162443          DOI： 10.1074/jbc.M111.250308

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  73 in total

1.  Contribution of the Na-K-Cl cotransporter on GABA(A) receptor-mediated presynaptic depolarization in excitatory nerve terminals.

Authors:  I S Jang; H J Jeong; N Akaike
Journal:  J Neurosci       Date:  2001-08-15       Impact factor: 6.167

2.  Identification of a chloride ion binding site in Na+/Cl -dependent transporters.

Authors:  Lucy R Forrest; Sotiria Tavoulari; Yuan-Wei Zhang; Gary Rudnick; Barry Honig
Journal:  Proc Natl Acad Sci U S A       Date:  2007-07-24       Impact factor: 11.205

3.  Patch-clamp and amperometric recordings from norepinephrine transporters: channel activity and voltage-dependent uptake.

Authors:  A Galli; R D Blakely; L J DeFelice
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-27       Impact factor: 11.205

4.  Measurement of transient currents from neurotransmitter transporters expressed in Xenopus oocytes.

Authors:  S Mager; Y Cao; H A Lester
Journal:  Methods Enzymol       Date:  1998       Impact factor: 1.600

5.  Antidepressant- and cocaine-sensitive human serotonin transporter: molecular cloning, expression, and chromosomal localization.

Authors:  S Ramamoorthy; A L Bauman; K R Moore; H Han; T Yang-Feng; A S Chang; V Ganapathy; R D Blakely
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-15       Impact factor: 11.205

6.  Serotonin and cocaine-sensitive inactivation of human serotonin transporters by methanethiosulfonates targeted to transmembrane domain I.

Authors:  L Keith Henry; Erika M Adkins; Qiao Han; Randy D Blakely
Journal:  J Biol Chem       Date:  2003-07-17       Impact factor: 5.157

7.  Mechanism of chloride interaction with neurotransmitter:sodium symporters.

Authors:  Elia Zomot; Annie Bendahan; Matthias Quick; Yongfang Zhao; Jonathan A Javitch; Baruch I Kanner
Journal:  Nature       Date:  2007-08-19       Impact factor: 49.962

8.  Amino acid residues that control pH modulation of transport-associated current in mammalian serotonin transporters.

Authors:  Y Cao; M Li; S Mager; H A Lester
Journal:  J Neurosci       Date:  1998-10-01       Impact factor: 6.167

9.  High affinity recognition of serotonin transporter antagonists defined by species-scanning mutagenesis. An aromatic residue in transmembrane domain I dictates species-selective recognition of citalopram and mazindol.

Authors:  E L Barker; M A Perlman; E M Adkins; W J Houlihan; Z B Pristupa; H B Niznik; R D Blakely
Journal:  J Biol Chem       Date:  1998-07-31       Impact factor: 5.157

10.  Molecular basis of alternating access membrane transport by the sodium-hydantoin transporter Mhp1.

Authors:  Tatsuro Shimamura; Simone Weyand; Oliver Beckstein; Nicholas G Rutherford; Jonathan M Hadden; David Sharples; Mark S P Sansom; So Iwata; Peter J F Henderson; Alexander D Cameron
Journal:  Science       Date:  2010-04-23       Impact factor: 47.728
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  25 in total

1.  Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog.

Authors:  Sotiria Tavoulari; Eleonora Margheritis; Anu Nagarajan; David C DeWitt; Yuan-Wei Zhang; Edwin Rosado; Silvia Ravera; Elizabeth Rhoades; Lucy R Forrest; Gary Rudnick
Journal:  J Biol Chem       Date:  2015-11-18       Impact factor: 5.157

2.  Hydrophobic plug functions as a gate in voltage-gated proton channels.

Authors:  Adam Chamberlin; Feng Qiu; Santiago Rebolledo; Yibo Wang; Sergei Y Noskov; H Peter Larsson
Journal:  Proc Natl Acad Sci U S A       Date:  2013-12-30       Impact factor: 11.205

3.  The second sodium site in the dopamine transporter controls cation permeation and is regulated by chloride.

Authors:  Lars Borre; Thorvald F Andreassen; Lei Shi; Harel Weinstein; Ulrik Gether
Journal:  J Biol Chem       Date:  2014-07-25       Impact factor: 5.157

4.  Computational modeling of human dopamine transporter structures, mechanism and its interaction with HIV-1 transactivator of transcription.

Authors:  Yaxia Yuan; Xiaoqin Huang; Jun Zhu; Chang-Guo Zhan
Journal:  Future Med Chem       Date:  2016-10-14       Impact factor: 3.808

Review 5.  Monoamine transporter structure, function, dynamics, and drug discovery: a computational perspective.

Authors:  Sankar Manepalli; Christopher K Surratt; Jeffry D Madura; Tammy L Nolan
Journal:  AAPS J       Date:  2012-08-24       Impact factor: 4.009

Review 6.  Kinase-dependent Regulation of Monoamine Neurotransmitter Transporters.

Authors:  Daniel P Bermingham; Randy D Blakely
Journal:  Pharmacol Rev       Date:  2016-10       Impact factor: 25.468

7.  The two Na+ sites in the human serotonin transporter play distinct roles in the ion coupling and electrogenicity of transport.

Authors:  Bruce Felts; Akula Bala Pramod; Walter Sandtner; Nathan Burbach; Simon Bulling; Harald H Sitte; L Keith Henry
Journal:  J Biol Chem       Date:  2013-11-29       Impact factor: 5.157

8.  Computational and biochemical docking of the irreversible cocaine analog RTI 82 directly demonstrates ligand positioning in the dopamine transporter central substrate-binding site.

Authors:  Rejwi Acharya Dahal; Akula Bala Pramod; Babita Sharma; Danielle Krout; James D Foster; Joo Hwan Cha; Jianjing Cao; Amy Hauck Newman; John R Lever; Roxanne A Vaughan; L Keith Henry
Journal:  J Biol Chem       Date:  2014-08-31       Impact factor: 5.157

9.  Ion-controlled conformational dynamics in the outward-open transition from an occluded state of LeuT.

Authors:  Chunfeng Zhao; Sebastian Stolzenberg; Luis Gracia; Harel Weinstein; Sergei Noskov; Lei Shi
Journal:  Biophys J       Date:  2012-09-05       Impact factor: 4.033

Review 10.  Current state of theoretical and experimental studies of the voltage-dependent anion channel (VDAC).

Authors:  Sergei Yu Noskov; Tatiana K Rostovtseva; Adam C Chamberlin; Oscar Teijido; Wei Jiang; Sergey M Bezrukov
Journal:  Biochim Biophys Acta       Date:  2016-03-03
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