BACKGROUND: Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. METHODS: To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBD patients and animal models. RESULTS: Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80%, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin-10⁻/⁻ mice, but not dextran sulfate sodium colitis mice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10⁻/⁻ mice. CONCLUSIONS: FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.
BACKGROUND: Host-intestinal microbial interaction plays an important role in the pathogenesis of inflammatory bowel diseases (IBDs). The surface molecules of the intestinal epithelium act as receptors for bacterial adhesion and regulate the intestinal bacteria. Some known receptors are the mucosal blood type antigens, which are regulated by the fucosyltransferase2 (FUT2) gene, and individuals who express these antigens in the gastrointestinal tract are called secretors. Recent research has revealed that the FUT2 gene is associated with Crohn's disease (CD) in western populations. METHODS: To clarify the contribution of mucosal blood type antigens in IBD, we determined the incidence of five previously reported single-nucleotide polymorphisms of the FUT2 gene in Japanese patients. We also used immunohistochemistry to investigate the antigen expression in mucosal specimens from IBDpatients and animal models. RESULTS: Genetic analysis revealed that all of the patients with colonic CD were secretors, whereas the incidence of secretors was 80, 80, 67, and 80%, respectively, for the control, ileocolonic CD, ileal CD, and ulcerative colitis groups (P = 0.036). Abnormal expression of blood type antigens was observed only in colonic CD. Interleukin-10⁻/⁻ mice, but not dextran sulfate sodiumcolitismice, had enhanced colonic expression of blood type antigens, and the expression of these antigens preceded the development of colitis in the interleukin-10⁻/⁻ mice. CONCLUSIONS:FUT2 secretor status was associated with colonic-type CD. This finding, taken together with the immunohistochemistry data, suggests that the abnormal expression of blood type antigens in the colon may be a unique and essential factor for colonic CD.
Authors: Dermot P B McGovern; Michelle R Jones; Kent D Taylor; Kristin Marciante; Xiaofei Yan; Marla Dubinsky; Andy Ippoliti; Eric Vasiliauskas; Dror Berel; Carrie Derkowski; Deb Dutridge; Phil Fleshner; David Q Shih; Gil Melmed; Emebet Mengesha; Lily King; Sheila Pressman; Talin Haritunians; Xiuqing Guo; Stephan R Targan; Jerome I Rotter Journal: Hum Mol Genet Date: 2010-06-22 Impact factor: 6.150
Authors: Marina Aspholm-Hurtig; Giedrius Dailide; Martina Lahmann; Awdhesh Kalia; Dag Ilver; Niamh Roche; Susanne Vikström; Rolf Sjöström; Sara Lindén; Anna Bäckström; Carina Lundberg; Anna Arnqvist; Jafar Mahdavi; Ulf J Nilsson; Billie Velapatiño; Robert H Gilman; Markus Gerhard; Teresa Alarcon; Manuel López-Brea; Teruko Nakazawa; James G Fox; Pelayo Correa; Maria Gloria Dominguez-Bello; Guillermo I Perez-Perez; Martin J Blaser; Staffan Normark; Ingemar Carlstedt; Stefan Oscarson; Susann Teneberg; Douglas E Berg; Thomas Borén Journal: Science Date: 2004-07-23 Impact factor: 47.728
Authors: Matam Vijay-Kumar; Catherine J Sanders; Rebekah T Taylor; Amrita Kumar; Jesse D Aitken; Shanthi V Sitaraman; Andrew S Neish; Satoshi Uematsu; Shizuo Akira; Ifor R Williams; Andrew T Gewirtz Journal: J Clin Invest Date: 2007-12 Impact factor: 14.808
Authors: Luca Maroni; Stan F J van de Graaf; Simon D Hohenester; Ronald P J Oude Elferink; Ulrich Beuers Journal: Clin Rev Allergy Immunol Date: 2015-06 Impact factor: 8.667
Authors: Pirjo Wacklin; Jarno Tuimala; Janne Nikkilä; Harri Mäkivuokko; Noora Alakulppi; Pia Laine; Mirjana Rajilic-Stojanovic; Lars Paulin; Willem M de Vos; Jaana Mättö Journal: PLoS One Date: 2014-04-14 Impact factor: 3.240